Effects of Adding Catalase in Diets on Growth Performance,Antioxidant Activity,Immune Performance and Intestinal Flora Structure of Macrobrachium nipponense
This experiment was conducted to investigate the effects of adding catalase(CAT)in diets on growth performance,antioxidant activity,immune performance and intestinal flora structure of Macrobrachium nipponense.Six diets were formulated with 0(CAT1 group,control group),75(CAT2 group),150(CAT3 group),300(CAT4 group),600(CAT5 group)and 1 200 U/kg of CAT(CAT6 group)in basal diets,and fed a total of 1 080 M.nipponense with an initial body weight of(0.10±0.03)g,3 replicates in each group and 60 in each replicate.The experiment lasted for 8 weeks.The results showed as follows:1)there was no significant difference in the survival rate of prawns among different groups(P＞0.05).However,the weight gain rate of M.nipponense was significantly higher than that of the control group with the addition of 150 U/kg of CAT in diets(P＜0.05).2)No significant difference of the hepatopancreatic superoxide dismutase(SOD)activity of prawns was observed in all groups(P＞0.05).The malondialdehyde(MDA)content in the CAT2,CAT3 and CAT4 groups was significantly lower than that in other groups(P＜0.05).The activity of CAT showed a tendency to increase and then to decrease,and its activity reached the highest in the CAT2 group.Meanwhile,polyphenol oxidase(PPO)activity in the hepatopancreas of M.nipponense showed a trend of in-creasing and then decreasing with the addition of dietary CAT,and the PPO activity of the prawn in the CAT3 and CAT4 groups was significantly higher than that in the CAT5 group(P＜0.05).Alkaline phosphatase(AKP)activity fluctuated to a certain extent,and the AKP activity in the CAT3 group was significantly higher than that of the CAT1,CAT2 and CAT4 groups(P＜0.05).3)There was no significant difference in the al-pha diversity of intestinal flora among all groups of M.nipponense(P＞0.05).After analyzing the beta diversi-ty of the intestine of M.nipponense,it was found that at the phylum level,the dominant bacteria in the intesti-nal tract were Proteobacteria,Firmicutes and Cyanobacteria,and that the relative abundance of Proteobacteria in the CAT3 and CAT4 groups was significantly lower than in the control group(P＜0.05),while the relative abundance of Firmicutes in the CAT3 and CAT4 groups was significantly higher than that in the control group(P＜0.05).At the genus level,the dominant genera were Tyzzerella,Aeromonas and Acinetobacter,and the relative abundance of Tyzzerella was significantly higher in the CAT4 group than in the control group(P＜0.05),while the relative abundance of Aeromonas was significantly lower in the CAT3,CAT4 and CAT5 groups than in the control group(P＜0.05).In conclusion,the addition of 150 to 300 U/kg CAT to the diets can improve the growth performance,antioxidant activity and immune performance,increase the abundance of beneficial intestinal flora,and promote the healthy cultivation of M.nipponense.[Chinese Journal of Animal Nutrition,2024,36(1):464-477]
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维普
