Lactulose and Bacillus coagulans Synbiotic Alleviates Lipopolysaccharide-Induced Intestinal Inflammation in Weaned Piglets through Toll-Like Receptor 4/Myeloid Differentiation Factor 88/Nuclear Factor-κB Signaling Pathway
The aim of this study was to investigate the molecular mechanism of the alleviating effects of lactu-lose and Bacillus coagulans synbiotic on intestinal inflammation induced by lipopolysaccharide(LPS)in weaned piglets.A total of 24 healthy Duroc×Landrace×Yorkshire crossbred weaned boars aged of 27 to 28 days with similar body weight were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate.Piglets in the control group(CON group)and LPS group were fed a basal diet,those in the chloro-tetracycline group(CTC group)were fed the basal diet+75 mg/kg chlorotetracycline,and those in the synbi-otic group(SYN group)were fed the basal diet+10 g/kg lactulose and 2×109 CFU/kg Bacillus coagulans,re-spectively.The experiment lasted for 32 days.At the end of the experiment,piglets in LPS group,CTC group and SYN group were intraperitoneally injected with 100 µg/kg BW LPS,and those in CON group were injec-ted with the same dose of normal saline.The serum and jejunal mucosa samples were collected and the contents of inflammatory cytokines and the expression of genes and proteins related to Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-KB(NF-κB)signaling pathway were determined.The results show as follows:1)compared with CON group,LPS stimulation not only significantly increased the contents of pro-inflammatory factors such as interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-12(IL-12),interferon-γ(IFN-γ)and tumor necrosis factor-α(TNF-α)as well as the anti-inflammatory factor as interleukin-10(IL-10)in serum,but also significantly increased the contents of pro-inflammatory factors such as IL-6,IL-12 and IFN-γ as well as anti-inflammatory factors such as interleu-kin-4(IL-4),IL-10 and transforming growth factor-β1(TGF-β1)in jejunal mucosa(P＜0.05).In addition,compared with CON group,LPS stimulation also significantly increased the mRNA relative expression levels of TNF-a,IL-1β and IL-10 in jejunal mucosa(P＜0.05),and significantly increased the mRNA and protein rela-tive expression levels of TLR4,MyD88 and NF-κB in jejunal mucosa(P＜0.05).2)Compared with LPS group,the contents of IL-1β and IL-10 in serum and contents of IL-6,IL-12,IL-4 and IL-10 in jejunal muco-sa in CTC group and SYN group were significantly decreased(P＜0.05),the TGF-β1 content in jejunal muco-sa was significantly increased(P＜0.05),the mRNA relative expression levels of TNF-α,IL-1β and IL-10 in jejunal mucosa were significantly decreased(P＜0.05),and the mRNA relative expression level of NF-κB and the protein relative expression levels of TLR4 and NF-κB in jejunal mucosa were significantly decreased(P＜0.05);the contents of IL-6,IL-8,IL-12 and TNF-α in serum and IL-1β content in jejunum mucosa in SYN group were significantly decreased(P＜0.05),and the mRNA relative expression levels of TLR4 and MyD88 and protein relative expression level of MyD88 in jejunum mucosa were significantly decreased(P＜0.05).In conclusion,dietary lactulose and Bacillus coagulans synbiotic instead of chlorotetracycline in antibiotic-free di-ets can inhibit the TLR4/MyD88/NF-KB signaling pathway in jejunal mucosa of weaned piglets,reduce the mRNA relative expression levels of TNF-α and IL-1β,improve the levels of inflammatory cytokines in jejunum and serum,and thus alleviate the LPS-induced stress response and improve the anti-stress ability of weaned pig-lets.[Chinese Journal of Animal Nutrition,2024,36(1):551-563]
万方数据
维普
