Study on Response Surface Method Optimization of Ultrasonic-Assisted Extraction Process Parameters for Gongying Powder and Its Antibacterial and Antioxidant Properties
The purpose of this experiment was to optimize the process parameters of ultrasonic-assisted extrac-tion of Gongying powder through response surface method,and to determine the in vitro antioxidant and anti-bacterial activity of aqueous extract of Gongying powder.The value range of each factor was determined by a single factor experiment,and three significant factors affecting the extraction rate of Gongying powder,such as ultrasonic temperature,ultrasonic time and ratio of liquid to material,were screened out by Plackett-Burman design,and then the optimum extraction conditions were obtained by Box-Behnken response surface analysis.The in vitro antibacterial activity was evaluated by microbroth dilution method and Kirby-Bauer disk diffusion method,and the in vitro antioxidant activity of aqueous extract of Gongying powder was investigated by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical scavenging methodand hydroxyl radical scavenging meth-od.The results showed that the extraction rate of Gongying powder was the highest when the ultrasonic power was 420 W,the ultrasonic temperature was 80 ℃,the ultrasonic time was 65 min and the ratio of liquid to ma-terial was 37∶1.The aqueous extract of Gongying powder had different inhibitory effects on three clinical patho-genic bacteria of dairy cow mastitis in vitro,the minimum inhibitory concentration(MIC)of Escherichia coli(E.coli)was 9.00 mg/mL,and the minimum bactericidal concentration(MBC)was 18.00 mg/mL;the MIC of Streptococcus agalactiae(S.agalactiae)was 4.50 mg/mL,and the MBC was 9.00 mg/mL;the MIC of Staphylococcus aureus(S.aureus)was 2.25 mg/mL,and the MBC was 4.50 mg/mL.In addition,when the concentration of aqueous extract of Gongying powder reached 1.00 mg/mL,it had good scavenging rates on hydroxyl radical(78.70％)and DPPH radical(75.63％)in vitro.In conclusion,the optimum extraction conditions of Gongying powder are successfully selected in this study,and the aqueous extract of Gongying powder has good in vitro antioxidant activity and different degrees of in vitro antibacterial activity against three clinical pathogenic bacteria of dairy cow mastitis.[Chinese Journal of Animal Nutrition,2024,36(1):579-591]
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维普
