Effects of Plant Essential Oil Combined with Tributyrin on Growth Performance,Blood Indices and Intestinal Morphology of Mice Challenged with Escherichia coli
This study was conducted to investigate the effects of plant essential oil combined with tributyrin on growth performance,immune function,antioxidant capacity and intestinal morphology of mice challenged with Escherichia coli.A total of 150 specific pathogen-free(SPF)Kunming(KM)female mice of 4-week-old with an initial body weight of(20±2)g were randomly divided into 5 groups with 3 replicates per group and 10 mice per replicate.Mice in the control group and challenge group were fed a basal diet,and those in the three experimental groups were fed the basal diet supplemented with 60 mg/kg plant essential oil+3.5 g/kg tributyrin(test group Ⅰ),80 mg/kg plant essential oil+3.5 g/kg tributyrin(test group Ⅱ)and 100 mg/kg plant essen-tial oil+3.5 g/kg tributyrin(test group Ⅲ)by intragastric administration,respectively.The experiment lasted for 21 days.On day 15 of the experiment,mice in the control group were intraperitoneally injected with phos-phate buffer(PBS)solution,and those in the other 4 groups were intraperitoneally injected with Escherichia coli culture solution(0.8xl08 CFU/mL).The results showed as follows:1)on days 1 to 7,compared with the control group,the average daily gain of mice in test group Ⅰ was significantly increased(P＜0.05);on days 15 to 21,compared with the challenge group,the average daily gain in test groups Ⅰ and Ⅱ was signifi-cantly increased(P＜0.05).2)On days 1,3,5 and 7 after challenge,compared with the control group,the challenge significantly increased the serum contents of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)(P＜0.05),and significantly decreased the serum contents of immunoglobulin A(IgA),immuno-globulin G(IgG),immunoglobulin M(IgM),interleukin-4(IL-4)and interleukin-10(IL-10)(P＜0.05);supplementation of plant essential oil combined with tributyrin significantly reduced the serum contents of IL-1βand TNF-α of challenged mice(P＜0.05),and significantly increased the serum contents of IgA,IgG,IgM,IL-4 and IL-10(P＜0.05).3)Compared with the control group,the challenge significantly decreased the ac-tivities of serum superoxide dismutase(SOD)and catalase(CAT)of mice(P＜0.05),and significantly in-creased the serum contents of malondialdehyde(MDA)and reactive oxygen species(ROS)(P＜0.05);sup-plementation of plant essential oil combined with tributyrin significantly increased the activities of SOD and CAT in serum of challenged mice(P＜0.05),and significantly decreased the serum contents of MDA and ROS(P＜0.05).4)Compared with the control group,the challenge significantly reduced the villus height and villus height to crypt depth ratio(V/C)in duodenum of mice(P＜0.05),and significantly increased the crypt depth(P＜0.05);supplementation of plant essential oil combined with tributyrin significantly increased the villus height and V/C in duodenum of challenged mice(P＜0.05),and significantly decreased the crypt depth(P＜0.05).In conclusion,under the challenge of Escherichia coli,supplementing plant essential oil combined with tributyrin can alleviate the inflammatory response and intestinal barrier damage of mice,maintain the intestinal health,improve the immune function and antioxidant capacity,and improve the growth performance.[Chinese Journal of Animal Nutrition,2024,36(1):592-601]
plant essential oiltributyrinimmune functionantioxidant capacityintestinal health
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维普
