Study on Mixed Silage of Pineapple Peel with Different Proportions of King Grass and Passion Fruit Vine
This experiment was conducted to investigate the effects of different mixing ratios of pineapple peel,king grass and passion fruit vine on fermentation quality and microbial diversity of silage.Mixed silage with pineapple peel,king grass and passion fruit vine were divided into 5 groups according to fresh weight ratios of 3∶6∶1,3∶5∶2,3∶4∶3,3∶3∶4 and 3∶2∶5,respectively.The fermentation quality,nutrient contents and microbial diversity of silage were analyzed after silage at room temperature for 40 days.The results showed as follows:1)with the increase of the mixed proportion of passion fruit vine,the pH and the contents of ammonia nitrogen(NH3-N)and butyric acid(BA)in silage showed an increasing trend,and the pH in 3∶6∶1 group was the lowest,which was significantly lower than that in the other groups except 3∶5∶2 group(P＜0.05);the contents of NH3-N and BA in 3∶6∶1 group were significantly lower than those in the other groups(P＜0.05).In addi-tion,the Flieg point evaluation of silage in all groups was good,and the Flieg point in 3∶6∶1 group was the highest.2)With the increase of the mixed proportion of passion fruit vine,the contents of dry matter(DM)and crude protein(CP)in silage showed an increasing trend,the DM content in 3∶2∶5 group was significantly higher than that in 3∶6∶1 and 3∶5∶2 groups(P＜0.05),and the CP content was significantly higher than that in the other groups except 3∶3∶4 group(P＜0.05);the contents of neutral detergent fiber(NDF)and acid deter-gent fiber(ADF)in silage showed a decreasing trend,the NDF content in 3∶6∶1 group was significantly higher than that in the other groups(P＜0.05),and the ADF content was significantly higher than that in 3∶3∶4 and 3∶2∶5 groups(P＜0.05).3)With the increase of the mixed proportion of passion fruit vine,the Shannon in-dex of microbial community in silage showed a decreasing trend,and the Shannon index in 3∶6∶1 group was significantly higher than that in 3∶2∶5 group(P＜0.05);the Ace index of microbial community in silage was firstly increased and then decreased,and the Ace index in 3∶5∶2 group was significantly higher than that in 3∶3∶4 and 3∶2∶5 groups(P＜0.05).After silage,the dominant bacteria in 3∶6∶1,3∶5∶2 and 3∶2∶5 groups at phylum and genus levels were Firmicutes and Lactobacillus,while the dominant bacteria in 3∶4∶3 and 3∶3∶4 groups at phylum and genus levels were Proteobacteria and Pantoea,respectively.In conclusion,the fermenta-tion effects on silage in 3∶6∶1 group is the best,and the recommended mixing ratio of pineapple peel,king grass and passion fruit vine is 3∶6∶1 in silage production.[Chinese Journal of Animal Nutrition,2024,36(1):637-649]
pineapple peelking grasspassion fruit vinemixed silagefermentation qualitymicrobial di-versity
万方数据
维普
