Effects of Euglena gracilis β-1,3-Glucan on Growth Performance,Serum Inflammatory Cytokine Contents and Intestinal Flora Structure of Weaned Piglets
The aim of this study was to investigate the effects of Euglena gracilis β-1,3-glucan on growth per-formance,serum inflammatory cytokine contents and intestinal flora structure of weaned piglets,and to provide evidence for the development and application of Euglena gracilis β-1,3-glucan.A total of 150 forty-day-old DurocxLandracexLarge White weaned piglets with similar body weight of(13.06±0.26)kg were randomly divided into 5 groups with 5 replicates per group and 6 pigs per replicate.The control group was fed a basal di-et,and the experimental groups were fed the basal diets supplemented with 50,100 and 150 mg/kg Euglena gracilis β-1,3-glucan and 100 mg/kg yeast β-1,3-glucan,respectively.The pre-experimental period lasted for 3 days,and the experimental period lasted for 28 days.The results showed as follows:1)compared with the control group,dietary 100 mg/kg Euglena gracilis β-1,3-glucan significantly increased the average daily feed intake and average daily gain(P＜0.05),and significantly decreased the ratio of feed to gain(P＜0.05).2)Compared with the control group,dietary 50,100 and 150 mg/kg Euglena gracilis β-1,3-glucan and 100 mg/kg yeast β-1,3-glucan significantly increased the contents of interleukin-1,interleukin-6 and tumor necrosis factor-α in serum(P＜0.05).3)Compared with the control group,dietary 50 mg/kg Euglena gracilis β-1,3-glucan significantly increased the Tenericutes relative abundance(P＜0.05),dietary 50 and 100 mg/kg Euglena gracilis β-1,3-glucan significantly increased the Lactobacillaceae relative abundance(P＜0.05),and dietary 50 and 150 mg/kg Euglena gracilis β-1,3-glucan significantly decreased the Ruminococcus relative a-bundance(P＜0.05).4)Compared with the control group,dietary 100 mg/kg Euglena gracilis β-1,3-glucan significantly increased the Chao1 index(P＜0.05),and dietary 150 mg/kg Euglena gracilis β-1,3-glucan sig-nificantly decreased the Simpson index(P＜0.05).The Beta diversity analysis showed that dietary 100 mg/kg Euglena gracilis β-1,3-glucan group had the most species similarity to the control group.In conclusion,dietary appropriate Euglena gracilis β-1,3-glucan can promote growth performance,reduce inflammatory reaction,and improve intestinal flora diversity and structure of weaned piglets.Under the conditions of this experiment,dietary 100 mg/kg Euglena gracilis β-1,3-glucan has the best effect.[Chinese Journal of Animal Nutrition,2024,36(3):1588-1598]
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