Study of Relationships between Eggshell Quality and Elementary Composition,Matrix Content and Ultrastructure of Longyan Shanma Ducks
The aim of this experiment was to investigate the effects of eggshell elementary composition,ultra-structure,and calcified eggshell organic matrix content on the eggshell quality of Longyan Shanma duck.In the experiment,three hundred eggs laid by 300-day-old Longyan Shanma ducks were selected and divided into three experimental groups of high,middling and low intensity according to the eggshell strength,with five rep-licates in each group and 20 eggs in each replicate.And the eggshell quality,elementary composition and ultra-structure were determined.The results showed that the eggshell strength values of the high,middling,and low intensity groups were(4.67±0.09)kg/cm2,(3.78±0.02)kg/cm2,and(2.86±0.03)kg/cm2,respectively,and the difference was highly significant(P<0.05).There were no significant differences in egg weight,egg shape index,eggshell thickness,and eggshell weight among the groups(P>0.05).There was no significant difference in the matrix content of calcified shell among the three groups(P>0.05).And there was no signifi-cant difference in the relative content of eggshell elements among the three groups at different levels(P>0.05).The ultrastructure of eggshell showed that compared with low and middling strength eggshell,high strength eggshell had better cross-sectional densification and closer connection at the top of mastoid.And there were significant differences in the effective thickness of mastoid layer among all groups(P<0.05),and signifi-cant differences in the mastoid gap between high and low strength groups(P<0.05).In conclusion,for egg-shells with similar thickness,the relative content of elements in each eggshell layer and the matrix content have no significant effect on the eggshell strength of Longyan Shanma ducks.The ultrastructure density of eggshell play an important role in the eggshell quality,and the effective thickness of mastoid layer and mastoid gap are the important factors affecting the eggshell quality.[Chinese Journal of Animal Nutrition,2024,36(3):1665-1673]