摘要
本试验旨在研究沙葱乙醇提取物对奶牛血浆生化、免疫、抗氧化和激素指标的影响.选取12头体况和产奶量相近、2～3胎次的泌乳中期荷斯坦奶牛,随机分为2组,每组6个重复,每个重复1头.对照组奶牛饲喂基础饲粮,试验组奶牛饲喂基础饲粮+54 g/d沙葱乙醇提取物.预试期14 d,正试期42 d.结果表明:1)2组之间血浆葡萄糖(GLU)、尿素(UREA)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、肌酐(CR)、胆固醇(CHOL)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性均无显著差异(P＞0.05).2)试验组的血浆谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GSH-Px)活性显著高于对照组(P＜0.05),血浆超氧化物歧化酶(SOD)活性极显著高于对照组(P＜0.01).2组之间血浆总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量均无显著差异(P＞0.05).3)试验组的血浆白细胞介素-2(IL-2)和白细胞介素-10(IL-10)含量显著高于对照组(P＜0.05),血浆白细胞介素-6(IL-6)含量极显著低于对照组(P＜0.01).2组之间血浆免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)含量均无显著差异(P＞0.05).4)2组之间血浆生长激素(GH)、催乳素(PRL)、雌二醇(E2)、胰岛素(INS)、皮质醇(HYD)含量均无显著差异(P＞0.05).由此可见,饲粮中添加54 g/d沙葱乙醇提取物能增强泌乳中期奶牛的免疫、抗氧化能力,且对奶牛血浆生化指标无不良影响.
Abstract
This experiment was conducted to study the effects of ethanol extract of Allium mongolicum Regel on plasma biochemical,antioxidant,immune and hormone indexes of dairy cows.Twelve 2 to 3 parity mid-lactation Holstein cows with similar body condition and milk production were selected and randomly divided in-to 2 groups with 6 replicates in each group and 1 cow in each replicate.Cows in the control group were fed a basal diet,and others in the test group were fed the basal diet+54 g/d ethanol extract of Allium mongolicum Regel.The pre-experimental period lasted for 14 days,and the experimental period lasted for 42 days.The re-sults showed as follows:1)there were no significant differences in glucose(GLU),urea(UREA),total protein(TP),albumin(ALB),globulin(GLB)contents and alanine aminotransferase(ALT),aspartate aminotransferase(AST)alkaline phosphatase(ALP)activities in plasma between 2 groups(P＞0.05).2)The glutathione(GSH)content and glutathione peroxidase(GSH-Px)activity in plasma of the test group were significantly higher than those of the control group(P＜0.05),and the plasma superoxide dismutase(SOD)activity was significantly higher than that of the control group(P＜0.01).There were no significant differences in total antioxidant capacity(T-AOC),catalase(CAT)activity and malondialdehyde(MDA)content in plasma between 2 groups(P＞0.05).3)The contents of interleukin-2(IL-2)and interleukin-10(IL-10)in plasma of the test group were significantly higher than those of the control group(P＜0.05),and the plasma interleukin-6(IL-6)content was significantly lower than that of the control group(P＜0.01).There were no significant differences in contents of immunoglobulin A(IgA),immunoglobulin M(IgM),immunoglobulin G(IgG),interferon-γ(IFN-γ)and tumor necrosis factor-α(TNF-α)in plasma between 2 groups(P＞0.05).4)There were no significant differences in contents of growth hormone(GH),prolactin(PRL),es-tradiol(E2),insulin(INS),cortisol(HYD)in plasma between 2 groups(P＞0.05).In conclusion,dietary adding 54 g/d ethanol extract of Allium mongolicum Regel can enhance the immune and antioxidant capacity of dairy cows during mid-lactation period,and has no adverse effects on the plasma biochemical indexes of dairy cows.[Chinese Journal of Animal Nutrition,2024,36(3):1734-1744]
基金项目
内蒙古农业大学青年教师科研能力提升专项(BR230102)
北京农学院奶牛营养学北京市重点实验室开放基金(2020019)
内蒙古农业大学动物科学学院高水平成果培育专项(QT202220)
维普
万方数据