Effects of Adding Bacillus coagulans into Diets with Different Concentrate to Forage Ratios on Rumen Fermentation and Immune Function of Lambs
The aim of this experiment was to investigate the effects of adding Bacillus coagulans(BC)into di-ets with different concentrate to forage ratios on rumen fermentation and immune function of lambs.A 2×2 two-factorial completely randomized design was used in the experiment,with different ratio of concentrate to forage(40∶60 of low-concentrate diet or 70∶30 of high-concentrate diet)and BC(adding 0 or 1.0 × 1010 CFU/kg BC)as two factors.Forty-eight Duper×small-tail Han sheep F1 male lambs with similar body weight and at the age of 4 months were selected and randomly divided into 4 groups with 12 lambs in each group.Lambs in LD,LD+BC,HD and HD+BC groups were fed low-concentrate diet,low-concentrate plus BC diet,high-concentrate diet and high-concentrate plus BC diet,respectively.the pre-test period was 10 days,and the trial period was 60 days.The results showed as follows:1)the pH,the concentrations of ammo-nia nitrogen,acetate,isovalerate and acetate/propionate ratio in the rumen of 24 lambs fed high-concentrate di-et were significantly lower than those of 24 lambs fed low-concentrate diet(P＜0.05),and the concentrations of propionate,valerate and total volatile fatty acids were significantly higher than those of 24 lambs fed low-concentrate diet(P＜0.05);the concentration of propionate in the rumen of 24 lambs fed adding BC diet was significantly higher than that of 24 lambs fed no BC diet(P＜0.05).There were no significant interactions be-tween dietary concentrate to forage ratio and BC on rumen fermentation parameters(P＞0.05).2)The activi-ties of amylase and protease in the rumen of 24 lambs fed high-concentrate diet were significantly higher than those of 24 lambs fed low-concentrate diet(P＜0.05),and the activity of xylanase was significantly lower than that of 24 lambs fed low-concentrate diet(P＜0.05).The counts of total bacteria,P.ruminicola and R.amylo-philus in the rumen of 24 lambs fed high-concentrate diet were significantly higher than those of 24 lambs fed low-concentrate diet(P＜0.05),and the counts of R.flavefaciens,F.succinicogenes,B.fibrisolven and pro-tozoa were significantly lower than those of 24 lambs fed low-concentrate diet(P＜0.05).The rumen amylase activity of 24 lambs fed adding BC diet was significantly higher than that of 24 lambs fed no BC diet(P＜0.05).The counts of total bacteria,R.amylophilus and P.ruminicola and B.fibrisolvens in the rumen of 24 lambs fed adding BC diet were significantly higher than those of 24 lambs fed no BC diet(P＜0.05).There were no significant interactions between dietary concentrate to forage ratio and BC in rumen microbial enzyme activities and functional microorganism counts of lambs(P＞0.05).3)The serum immunoglobulin G(IgG)content of 24 lambs fed high-concentrate diet was significantly lower than that of 24 lambs fed low-concentrate diet(P＜0.05).The serum interleukin-4(IL-4)content of 24 lambs fed adding BC diet was significantly high-er than that of 24 lambs fed no BC diet(P＜0.05).There was a significant interaction between dietary concen-trate to forage ratio and BC on serum IL-4 content of lambs(P＜0.05).In conclusion,adding BC into diets with different concentrate to forage ratios can promote rumen fermentation,effectively improve the digestion and absorption of the diet,and also improve the inflammatory response level of the body,thus promoting the healthy growth of lambs,and the effect of BC supplementation is better under high-concentrate feeding condi-tions.Therefore,BC can be used as a new probiotic preparation in lamb production,and the effect of adding BC to high-concentrate diet with concentrate to forage ratio of 70:30 is better.[Chinese Journal of Animal Nu-trition,2024,36(3):1769-1781]
lambsdifferent concentrate to forage ratio dietsBacillus coagulansrumen fermentationim-mune function
万方数据
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