Effects of Amino Acid Trace Element Chelates on Growth Performance,Serum Biochemical Indexes,and Trace Element Deposition of Grass Carp(Ctenopharyngodon idellus)
In order to study the effects of amino acid trace element chelates on the growth performance,trace element deposition and serum biochemical indexes of grass carp,a total of 720 grass carp with an initial body weight of(40.05±0.05)g were randomly divided into 6 groups.The control group(CON group)was fed a basal diet without trace elements,and the inorganic trace element addition group(IT100 group)was fed a basal diet supplemented with 100％inorganic trace elements copper,iron,zinc and manganese required by grass carp trace elements.The fish in the amino acid trace element chelate addition groups(OT25,OT50,OT75 and OT100 groups)were fed the experimental diets in which 25％,50％,75％and 100％amino acid trace element chelates were used to replace the inorganic trace elements in the diet of the IT100 group in equal proportions,with 3 replicates in each group and 40 fish in each replicate.The fish were fed for 8 weeks.Re-sults showed as follows:1)there was no significant difference in survival rate,condition factor,liver-body ra-tio and visceral-body ratio among different groups(P＞0.05).Compared with the CON group,the weight gain rate in the IT100 group and the OT75 group was significantly increased(P＜0.05),while the change trend of the feed coefficient was opposite;the OT50 group and the OT75 group were significantly lower than the CON group(P＜0.05).2)Compared with CON group,the activities of trypsin,amylase and lipase in IT100 group,OT50 group,OT75 group and OT100 group were significantly increased(P＜0.05),and the amylase activity in OT25 group was significantly increased(P＜0.05).3)According to the quadratic regression equa-tions for trace element deposition in grass carp vertebrae,the efficacy of amino acid trace element chelates reached 100％of that of inorganic trace elements when the addition levels were Cu 67.53％,Fe 58.22％,Zn 58.68％and Mn 56.07％.4)Compared with the CON group,the contents of serum total cholesterol,low den-sity lipoprotein cholesterol,urea nitrogen and the activities of alanine aminotransferase and aspartate amin-otransferase in the IT100 group were significantly decreased(P＜0.05),and the serum contents of complement 3,high density lipoprotein cholesterol and alkaline phosphatase were significantly decreased(P＜0.05).Com-pared with the CON group,the serum triglyceride content in OT groups was significantly decreased(P＜0.05),the serum total cholesterol,urea nitrogen content and alanine aminotransferase activity were significant-ly decreased in OT100 group(P＜0.05),the low density lipoprotein cholesterol content and aspartate amin-otransferase activity were significantly decreased in OT75 and OT100 groups(P＜0.05),and the alkaline phosphatase activity was significantly increased in OT100 group(P＜0.05).In conclusion,the appropriate sub-stitution of amino acid trace element chelates for inorganic trace elements in feed can improve the growth,di-gestion and absorption,as well as trace element deposition in the vertebrae of grass carp.Among them,75％a-mino acid trace element chelate substitution ratio was the optimal substitution ratio.[Chinese Journal of Animal Nutrition,2024,36(3):1806-1818]
grass carpamino acid trace element chelatesgrowth performancetrace element depositionse-rum biochemical index
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