Research on Improvement of Growth,Antioxidant Ability,Intestinal and Hepatic Function of Siniperca chuatsi by L-Carnitine
In order to investigate the effects of adding L-carnitine in diets on the growth,antioxidant ability,and intestinal and hepatic tissue and function of Siniperca chuatsi,a total of 270 fish with initial body weight of(53.98±1.32)g were divided into six groups(named as D0,D50,D100,D150,D200 and D250 groups)by adding L-carnitine at effective contents of 0,50,100,150,200 and 250 mg/kg into the basal diet of Sini-perca chuatsi,respectively.Each group consisted of three replicates with 15 fish per replicate for 56-day growth assays.Results showed as following:1)compared with D0 group,weight gain rate was significantly higher in the D100 group(P＜0.05),feed conversion ratio was significantly lower in the D100 group(P＜0.05);the weight gain rate in D250 group was significantly decreased(P＜0.05),and the feed conversion ra-tio was significantly increased(P＜0.05).2)Compared with D0 group,plasma alanine aminotransferase and aspartate aminotransferase activities were significantly lower than those in the D100 group(P＜0.05)and sig-nificantly higher in the D250 group(P＜0.05).3)Compared with D0 group,plasma triglyceride and total cholesterol contents were significantly lower in all test groups(P＜0.05);there was no significant difference in the contents of high-density lipoprotein cholesterol and low-density lipoprotein cholesterol among groups(P＞0.05),except that the content of low-density lipoprotein cholesterol in D100 and D150 groups decreased sig-nificantly(P＜0.05).4)Compared with D0 group,the activities of pepsin,trypsin,amylase,lipase and Na+-K+-ATPase were significantly increased in group D100(P＜0.05).5)With the rise of L-carnitine addition,D-lactic acid content and diamine oxidase activity first decreased and then increased,and reached the lowest val-ues in the D150 and D100 groups,respectively,which were significantly lower than those in the D0 group(P＜0.05),and endotoxin content was significantly decreased in all experimental groups(P＜0.05).6)Com-pared with D0 group,plasma and hepatic total oxidizing capacity was significantly increased(P＜0.05),and hepatic malondialdehyde content was significantly decreased in the D100 group(P＜0.05).In addition,im-mune globulin M content was significantly increased in all test groups(P＜0.05).7)Intestinal villus length and cuprocyte density were significantly higher in the D100 group compared with the D0 group(P＜0.05),and the intestinal changes were not significant in the D250 group(P＞0.05);myofibril diameters were significantly lower in the D100 and D250 groups(P＜0.05),and myofibril densities were significantly higher(P＜0.05),and the percentage of lipid droplets in hepatic tissue was significantly reduced compared with the D0 group(P＜0.05).In summary,L-carnitine can promote the development of Siniperca chuatsi's intestinal tract,enhance the ability of digestion and absorption,improve the growth rate,and at the same time,it has the effect of im-proving immunity and muscle quality,reduces hepatic fatty deposits.Based on weight gain rate and feed con-version ratio as indicators,the appropriate amount of L-carnitine added to the diet of Siniperca chuatsi is deter-mined to be 79.27 to 80.61 mg/kg.[Chinese Journal of Animal Nutrition,2024,36(3):1834-1845]
万方数据
维普
