Prolactin Improve Lactoferrin Synthesis and Secretion of Bovine Mammary Epithelial Cells through Janus Kinase 2/Signal Transducer and Activator of Transcription 5 Signaling Pathway
The aim of this experiment was to investigate the effects of prolactin(PRL)on the synthesis and se-cretion of lactoferrin(LF)mediated by the Janus kinase 2/signal transducer and activator of transcription 5(JAK2/STAT5)signaling pathway in bovine mammary epithelial cells(BMECs).BMECs were treated with different concentrations of PRL[0(control),10,100,1 000 ng/mL]for 24 h.The effects of PRL on LF content and the expression of LF,prolactin receptor(PRLR)and JAK2/STAT5 signaling pathway genes and related proteins were analyzed to verify the key role of STAT5 in prolactin-induced LF synthesis and secretion in BMECs.BMECs were treated with STAT5 inhibitor Pimozide[no Pimozide(control),10 µmol/L Pimo-zide,100 ng/mL PRL,and 10 µmol/L Pimozide+100 ng/mL PRL]24 h,effects of PRL and Pimozide on LF content and gene and related protein expression and relative fluorescence intensity of LF and JAK2/STAT5 signaling pathway were determined.The results showed as follows:1)compared with the control group,10,100,1 000 ng/mL PRL could significantly or very significantly increase the LF content in the cell supernatant of BMECs(P＜0.05 or P＜0.01);the Pimozide was able to significantly reduce the LF content in the cell su-pernatant of BMECs(P＜0.0l);the PRL was able to significantly increase the LF content in the supernatant of BMECs cells(P＜0.01).2)The relative expression levels of LF,PRLR,JAK2 and STAT5 genes in the 10 ng/mL PRL group were significantly increased(P＜0.05);the relative expression levels of LF,PRLR,JAK2 and STAT5 gene were very significantly higher in the 100 ng/mL PRL group(P＜0.01);the relative ex-pression levels of PRLR and STAT5 genes in the 1 000 ng/mL PRL group were significantly or extremely sig-nificantly increased(P＜0.05 or P＜0.01);the relative expression level of LF gene in the Pimozide group de-creased significantly(P＜0.05);the relative expression level of LF gene in BMECs in the PRL group was sig-nificantly increased(P＜0.05).3)The expression of LF protein in BMECs in the 100 and 1 000 ng/mL PRL groups gradually increased(P＜0.01);10,100,1 000 ng/mL PRL significantly or very significantly in-creased the expression of p-JAK2/JAK2 and p-STAT5/STAT5 in BMECs(P＜0.05 or P＜0.01);the Pimo-zide and the Pimozide+PRL significantly reduced the expression of LF protein in BMECs(P＜0.01).LF pro-tein expression was significantly increased in BMEC of PRL group(P＜0.01).4)The Pimozide and Pimozide+PRL significantly or very significantly reduced the relative fluorescence intensity of LF or p-STAT5 in BMECs(P＜0.05 or P＜0.01);the relative fluorescence intensity of LF and p-STAT5 in BMECs was very significantly increased in the PRL group(P＜0.01).In summary,PRL promotes PRL synthesis and secretion in BMECs cells of dairy cows through the JAK2/STAT5 signaling pathway.[Chinese Journal of Animal Nu-trition,2024,36(3):1916-1926]
万方数据
维普
