Effects of Tea Tree Oil on Growth Performance,Antioxidant Capacity,Immune Function and Intestinal Barrier Function of Weaned Piglets
This study aimed to investigate the effects of tea tree oil(TTO)on the growth performance,antiox-idant capacity,immune function and the intestinal barrier function of weaned piglets.One hundred and fifty weaned piglets at 21 days of age were selected and randomly divided into five groups,with three replicates in each group and 10 piglets in each replicate.Piglets in control group were fed a basal diet,while those in TTO groups were fed diets added 25,50,100 and 200 mg/kg TTO to the basal diet.The growth performance inde-xes,plasma antioxidant and immune indexes were measured after 28 days of feeding.And then the control group and 50 mg/kg TTO group were selected according to the results of growth performance and plasma an-tioxidant and immune indexes for the detection of intestinal mucosal antioxidant and immune indexes,hematox-ylin-eosin(HE)stained sections to observe the intestinal tissue structure,and 16S rDNA sequencing to analyze the structure of intestinal flora.The results showed as follows:1)the feed-to-gain ratio in all TTO groups were extremely significantly lower than those in the control group(P＜0.01),and there was no significant difference among the TTO groups(P＞0.05),but the lowest feed-to-gain ratio was found in the 50 mg/kg TTO group.2)Plasma glutathione peroxidase(GSH-Px)content was extremely significantly increased(P＜0.01)and ma-londialdehyde(MDA)content was extremely significantly decreased(P＜0.01)in the 25,50,100 and 200 mg/kg TTO groups compared with the control group.3)The plasma content of immunoglobulin G(IgG)in the 50,100 and 200 mg/kg TTO groups was extremely significantly higher than that in the control group(P＜0.01);the plasma content of immunoglobulin M(IgM)in the 25 and 200 mg/kg TTO groups was ex-tremely significantly higher than that in the control group(P＜0.01),and also the 100 mg/kg TTO group was significantly higher than the control group(P＜0.05).4)The ileal villi length/crypt depth ratio was extremely significantly elevated in the 50 mg/kg TTO group compared with the control group(P＜0.01).5)Compared with the control group,the content of reduced glutathione(GSH)in the mucosa of duodenum and ileum was extremely significantly increased(P＜0.01),the content of GSH-Px in the mucosa of duodenum and jejunum was extremely significantly increased(P＜0.01),the content of superoxide dismutase(SOD)in the mucosa of ileum was extremely significantly increased(P＜0.01),and the content of malondialdehyde(MDA)in the mucosa of jejunum and ileum was extremely significantly decreased(P＜0.01)in the 50 mg/kg TTO group.6)The content of secretory immunoglobulin A(sIgA)in the duodenal and ileal mucosa in the 50 mg/kg TTO group was extremely significantly higher than that in the control group(P＜0.01).7)At the phylum level,the relative abundance of Firmicutes of the duodenum in the 50 mg/kg TTO group was significantly higher than that in the control group(P＜0.05),and the relative abundance of Proteobacteria was significantly lower than that in the control group(P＜0.05);at the genus level,the relative abundance of Clostridium of the duodenum and ileum in the 50 mg/kg TTO group was extremely significantly higher than that in the control group(P＜0.01),and the relative abundance of Klebsiella of the duodenum,ileum and cecum was significantly lower than that in the control group(P＜0.05).The 50 mg/kg TTO group showed extremely significant enhancement in amino acid metabolism,metabolism of cofactors and vitamins,and metabolism of terpenoids and polyketides compared with the control group(P＜0.01).In conclusion,it can be seen that the addition of TTO to the diet can improve the growth performance of weaned piglets,enhance the antioxidant capacity and immune function of the body,strengthen the intestinal barrier function,and improve the health of piglets.[Chinese Journal of Animal Nutrition,2024,36(4):2246-2261]
tea tree oilweaned pigletsgrowth performanceantioxidant capacityintestinal barrier
万方数据
维普
