This experiment was conducted to study the effects of dietary docosahexaenoic acid(DHA)and glycerol monolaurate(GML)enriched complex on semen quality of boars.Forty-eight 19 to 24-month-old Du-roc boars were randomly divided into 4 groups with 12 replicates per group and 1 pig per replicate.Pigs in the control group were fed a basal diet,and others in experimental groups 1,2 and 3 were fed the basal diet sup-plemented with 0.18％,0.24％and 0.30％DHA and GML enriched complex,respectively.The experiment lasted for 16 weeks.The results showed as follows:1)the contents of DHA,n-3 polyunsaturated fatty acids and polyunsaturated fatty acids in sperm of experimental groups 1,2 and 3 were significantly higher than those of the control group(P＜0.01).2)On week 16,the total sperm count and effective sperm count of experi-mental groups 1,2 and 3 were significantly higher than those of the control group(P＜0.05),and the sperm abnormal rate was significantly lower than that of the control group(P＜0.01).3)On weeks 4,12 and 16,the sperm membrane integrity of experimental groups 1,2 and 3 was significantly higher than that of the con-trol group(P＜0.01);on week 10,the sperm membrane integrity of experimental groups 2 and 3 was signifi-cantly higher than that of the control group(P＜0.05).4)The rotational motile sperm ratio in fresh semen of experimental group 1 was significantly lower than that of the control group(P＜0.05);after stored 72 h,the rotational motile sperm ratio of experimental group 1 was significantly lower than that of the control group(P＜0.01).5)The serum malondialdehyde(MDA)content of experimental group 2 was significantly lower than that of the control group and experimental groups 1 and 3(P＜0.01).The fresh sperm superoxide dismutase(SOD)activity of experimental groups 1 and 2 was significantly higher than that of the control group(P＜0.01),and the fresh seminal plasma SOD activity of experimental group 2 was significantly higher than that of the control group(P＜0.05).After stored 24 h,the sperm MDA content of experimental groups 1,2 and 3 was significantly lower than that of the control group(P＜0.01).6)The serum interleukin-6(IL-6)content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.01).The fecal diam-ine oxidase(DAO)content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.01),and the fecal calprotectin content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.05).In conclusion,the DHA and GML enriched complex positively influ-ence the semen quality of boars by affecting the sperm fatty acid composition,oxidative stress and inflammato-ry status.[Chinese Journal of Animal Nutrition,2024,36(4):2262-2276]
维普
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