Effects of Dietary Docosahexaenoic Acid and Glycerol Monolaurate Enriched Complex on Semen Quality of Boars
This experiment was conducted to study the effects of dietary docosahexaenoic acid(DHA)and glycerol monolaurate(GML)enriched complex on semen quality of boars.Forty-eight 19 to 24-month-old Du-roc boars were randomly divided into 4 groups with 12 replicates per group and 1 pig per replicate.Pigs in the control group were fed a basal diet,and others in experimental groups 1,2 and 3 were fed the basal diet sup-plemented with 0.18％,0.24％and 0.30％DHA and GML enriched complex,respectively.The experiment lasted for 16 weeks.The results showed as follows:1)the contents of DHA,n-3 polyunsaturated fatty acids and polyunsaturated fatty acids in sperm of experimental groups 1,2 and 3 were significantly higher than those of the control group(P＜0.01).2)On week 16,the total sperm count and effective sperm count of experi-mental groups 1,2 and 3 were significantly higher than those of the control group(P＜0.05),and the sperm abnormal rate was significantly lower than that of the control group(P＜0.01).3)On weeks 4,12 and 16,the sperm membrane integrity of experimental groups 1,2 and 3 was significantly higher than that of the con-trol group(P＜0.01);on week 10,the sperm membrane integrity of experimental groups 2 and 3 was signifi-cantly higher than that of the control group(P＜0.05).4)The rotational motile sperm ratio in fresh semen of experimental group 1 was significantly lower than that of the control group(P＜0.05);after stored 72 h,the rotational motile sperm ratio of experimental group 1 was significantly lower than that of the control group(P＜0.01).5)The serum malondialdehyde(MDA)content of experimental group 2 was significantly lower than that of the control group and experimental groups 1 and 3(P＜0.01).The fresh sperm superoxide dismutase(SOD)activity of experimental groups 1 and 2 was significantly higher than that of the control group(P＜0.01),and the fresh seminal plasma SOD activity of experimental group 2 was significantly higher than that of the control group(P＜0.05).After stored 24 h,the sperm MDA content of experimental groups 1,2 and 3 was significantly lower than that of the control group(P＜0.01).6)The serum interleukin-6(IL-6)content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.01).The fecal diam-ine oxidase(DAO)content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.01),and the fecal calprotectin content of experimental groups 1 and 2 was significantly lower than that of the control group(P＜0.05).In conclusion,the DHA and GML enriched complex positively influ-ence the semen quality of boars by affecting the sperm fatty acid composition,oxidative stress and inflammato-ry status.[Chinese Journal of Animal Nutrition,2024,36(4):2262-2276]
万方数据
维普
