Effects of Alginate Oligosaccharide on Growth Performance,Immune Organ Indices,Serum Antioxidant Indices and Intestinal Health of Broilers
The aim of this study was to reveal the effects of alginate oligosaccharide(AOS)on growth per-formance,immune organ indices,serum antioxidant indices and intestinal health of broilers.Sodium alginate was hydrolyzed to prepare AOS of different molecular weights with alginate lyase PDE9 at enzyme-activity u-nits of 100,200 and 800 U.The molecular weights of the prepared AOS were determined using high perform-ance molecular exclusion chromatography.The weight-average molecular weights of the three AOS were 23 981,5 038 and 3 766 u,which were sequentially labeled with high molecular weight,medium molecular weight and low molecular weight,respectively.Then a total of 280 male Arbor Acres(AA)broiler chickens of 1-day-old with the same body condition and similar body weight were randomly divided into 4 groups with 7 replicates per group and 10 chickens per replicate.Broilers in the control group(CON group)were fed a basal diet,and the others in experiment groups were fed the basal diet supplemented with 200 mg/kg AOS with low molecular weight(AL group),medium molecular weight(AM group)and high molecular weight(AH group),respectively.The experiment lasted for 40 days.The results showed as follows:1)compared with CON group,the body weight of broilers at 40 days of age and the average daily gain(ADG)during 1 to 40 and 21 to 40 days of age in AL,AM and AH groups were significantly increased(P＜0.05);the body weight of broilers at 20 days of age and ADG during 1 to 20 days of age in AL and AM groups were significantly in-creased(P＜0.05),and the ratio of feed to gain(F/G)during 1 to 20 days of age was significantly decreased(P＜0.05).2)Compared with CON group,the liver index of broilers at 20 days of age in AL group was sig-nificantly decreased(P＜0.05);the bursa of Fabricius index of broilers at 20 days of age in AM and AH groups was significantly increased(P＜0.05).3)Compared with CON group,the crypt depth(CD)in jeju-num of broilers in AH group tended to be decreased(P=0.084),the mRNA relative expression levels of sodi-um-glucose cotransporter 1(SGLT1),zinc transporter 1(ZnT1),zonula occluden-1(ZO-1),mucin-2,oc-cludin in AH group were significantly increased(P＜0.05),and the claudin-3 mRNA relative expression level in jejunum tended to be increased(P=0.081).4)Compared with CON group,the mRNA relative expression levels of interleukin-10(IL-10),interleukin-17(IL-17)and interleukin-1 β(IL-1β)in cecal tonsil of broilers in AH group were significantly decreased(P＜0.05).5)Compared with CON group,the β diversity of micro-biota in cecum of broilers in AH group tended to be increased(P=0.055),and the relative abundances of Bacteroides,Ruminococcaceae_UCG-005,Ruminiclostridium,Phascolarctobacterium,Parasutterella,Campylobacter,Gallibacterium and Escherichia-Shigella in cecum in AH group were significantly increased(P＜0.05).In conclusion,adding different molecular weights of AOS in diets can effectively improve the growth performance of broilers;among them,the high molecular weight AOS requires less enzyme in prepara-tion,has relatively lower production cost,and can enhance immune function and improve intestinal health of broilers.[Chinese Journal of Animal Nutrition,2024,36(4):2331-2343]
alginate oligosaccharidebroilersgrowth performanceimmune organ indicesserum antioxidant indicesintestinal health
万方数据
维普
