Effects of Different Proportions of Wheat Supplementation on Performance,Egg Quality,Serum Biochemical Indices,Small Intestine Morphology and Cecal Microflora of Fengda No.1 Laying Hens During Peak Laying Period
This experiment was conducted to investigate the effects of different proportions of wheat supple-mentation on performance,egg quality,serum biochemical indices,small intestine morphology and cecal mi-croflora of Fengda No.1 laying hens during peak laying period.A total of 768 Fengda No.1 laying hens of 300-day-old with good growth and similar body condition were randomly divided into 4 groups with 6 replicates in each group and 32 hens in each replicate.Hens in the control group were fed a corn-soybean meal basal type diet,while those in the experimental groups were fed diets supplemented with 20%,40%and 60%wheat,re-spectively,which were based on the dietary nutrient levels in the control group,and supplemented with an ap-propriate proportion of compound wheat enzyme according to the wheat dosage.Meanwhile,the diets were ad-justed accordingly to ensure the basic consistency of dietary energy and protein levels.The pre-experimental pe-riod lasted for 7 days and the trial period lasted for 49 days.The results showed as follows:1)compared with the control group,the supplementation of 20%,40%and 60%wheat had no significant effects on the main performance indices of laying hens during peak laying period(P>0.05).2)Compared with the control group,the supplementation of different proportions of wheat significantly reduced the yolk color(P<0.05),and sig-nificantly increased the eggshell strength and eggshell thickness(P<0.05).3)Compared with the control group,the supplementation of 40%and 60%wheat significantly increased the serum calcium content(P<0.05),and significantly decreased the serum triglyceride content(P<0.05);meanwhile,the serum high den-sity lipoprotein cholesterol content in 40%wheat group was significantly higher than that in the other three groups(P<0.05),and the serum albumin content in 60%wheat group was significantly higher than that in the other three groups(P<0.05).4)Compared with the control group,the supplementation of different propor-tions of wheat had no significant effects on villus height,crypt depth and the ratio of villus height to crypt depth in small intestine of laying hens during peak laying period(P>0.05).5)Compared with the control group,there was no significant difference in α diversity and β diversity of cecal microflora in 40%wheat group(P>0.05);at the phylum level,the supplementation of 40%wheat significantly increased the relative abundances of Desulfobacterota and WPS-2 in cecum(P<0.05).In conclusion,the supplementation of different propor-tions of wheat can increase the eggshell strength and eggshell thickness,decrease the serum lipid content,and improve nutritional metabolism of Fengda No.l laying hens during peak laying period.Under the conditions of this experiment,it is more appropriate to add 40%wheat in the diet for Fengda No.1 laying hens during peak laying period.[Chinese Journal of Animal Nutrition,2024,36(4):2356-2369]