Effects of Biotin on Serum Biochemical Indices,Intestinal Mucosal Epithelial Morphology and Intestinal Flora Structure of Breeding Geese during Laying Period
The aim of this experiment was to study the effects of biotin on serum biochemical indices,intestinal mucosal epithelial morphology and intestinal flora structure of breeding geese during laying period.A total of 150 thirty-four-week-old Wulong geese(open-eyed geese)breeders with similar size were randomly divided into 6 groups with 5 replicates in each group and 5 geese(1 male and 4 females)in each replicate.The dietary biotin supplemental levels of 6 groups were 0(control),0.1,0.2,0.3,0.4 and 0.5 mg/kg,respectively.The pre-test period was 1 week,and the test period was 17 weeks.The results showed as follows:1)dietary biotin supplemental levels had no significant effects on the contents of total serum cholesterol,low density lipoprotein cholesterol and triglyceride in serum(P＞0.05).The serum total protein content of 0.2,0.3 and 0.4 mg/kg bi-otin groups was significantly higher than that of the control group(P＜0.05),the serum albumin content of 0.1,0.2,0.3 and 0.5 mg/kg biotin groups was significantly higher than that of the control group(P＜0.05),and the serum high density lipoprotein cholesterol content of 0.2,0.3,0.4 and 0.5 mg/kg biotin groups was significantly higher than that of the control group(P＜0.05).2)The duodenal villus height of 0.2,0.3,0.4 and 0.5 mg/kg biotin groups was significantly higher than that of the control group(P＜0.05),and the duode-nal villus height of 0.2 mg/kg biotin group was significantly higher than that of other groups(P＜0.05).The duodenal villus height/crypt depth of 0.3 mg/kg biotin group was significantly higher than of other groups ex-cept 0.2 mg/kg biotin group(P＜0.05).Dietary biotin supplemental levels had no significant effect on the duo-denal crypt depth(P＞0.05).3)Compared with the control group,the relative abundances of Massilia and Pygmaiobacter in cecum of 0.2 mg/kg biotin group were significantly increased(P＜0.05),and the relative a-bundances of Romboutsia,Turicibacter and Flavonifractor in cecum were significantly decreased(P＜0.05).In conclusion,dietary supplemented appropriate level of biotin in can promote the morphological development of intestinal mucosal epithelium and regulate the of intestinal flora structure of breeding geese during laying pe-riod.Under the conditions of this experiment,the dietary supplemented appropriate level of biotin for breeding geese during laying period is 0.26 to 0.34 mg/kg.[Chinese Journal of Animal Nutrition,2024,36(4):2395-2405]
breeding geese during laying periodbiotinserum biochemical indicesmucosal epithelial mor-phologyflora structure
万方数据
维普
