Effects of Basal Diet Replaced by Fermented Feed Mulberry with Different Proportions on Rumen Fermentation,Rumen Bacterial and Fungal Community Structure of Beef Cattle
This study was conducted to explore the effects of basal diet replaced by fermented feed mulberry with different proportions on rumen fermentation,rumen bacterial and fungal community structure of beef cat-tle.A total of 30 Simmental crossbred fattening beef cattle with similar body weight(287.45±22.63)kg were randomly divided into 3 group,namely the control group,the NT1 and NT2 groups,with 10 replicates per group.The control group was fed a basal diet,NT1 and NT2 groups were fed experimental diets which used 10％and 20％fermented feed mulberry to replace the basal diet.The pre-test lasted for 14 days,and the test lasted for 116 days.The results showed as follows:1)the content of pentanoic acid in rumen fluid in NT1 and NT2 groups significantly increased compared with the control group(P＜0.05).2)Compared with the control group,the Firmicutes/Bacteroidetes ratio(F/B)in the NT2 group significantly increased(P＜0.05),the rela-tive abundance of Prevotella significantly reduced(P＜0.05),the relative abundance of unclassified_Oscil-lospiraceae and Lachnospiraceae_UCG-008 significantly increased(P＜0.05).Moreover,the rumen bacteria in NT2 group enriched in the pathways:environmental information processing,signal transduction,two-compo-nent system,drug resistance:antimicrobial significantly increased(P＜0.05),and the pathways:global and o-verview maps,biosynthesis of other secondary metabolites,biosynthesis of antibiotics significantly decreased(P＜0.05)compared with the control group.3)The observed_species,ACE,Chao1,and Shannon indexes of fungal in the NT1 group were significantly higher than those in the control group(P＜0.05).In addition,com-pared with the control group,the relative abundance of Penicillium,Botryotinia,Byssochlamys,and Tri-chodermas in the NT1 group significantly upregulated(P＜0.05 or P＜0.01),while the relative abundance of Rhodotorula,Conoxybe and unclassified_Ceratobasidaceae significantly downregulated(P＜0.05 or P＜0.01).Simultaneously,the relative abundance of Rhodotorula,Conoxybe and unclassified_Ceratobasidaceae in the NT2 group also decreased compared wiht the control group(P＜0.05).In summary,different proportions of diet replaced by fermented feed mulberry increase the content of pentanoic acid in rumen fluid,increase the di-versity of rumen fungi flora,alter the composition and function of rumen bacteria and fungi,thus improve ru-men internal environment.[Chinese Journal of Animal Nutrition,2024,36(4):2475-2486]
fermented feed mulberrybeef cattlerumen fermentation parametersmicrobial community structure
万方数据
维普
