Effects of Replacing Soybean Meal with Milk Thistle Meal on Growth Performance,Blood Biochemical Indexes,Liver Antioxidant Indexes and Metabolism Related Gene Expression of Lambs
The main purpose of this experiment was to investigate the effects of replacing soybean meal with milk thistle meal on the growth performance,blood biochemical indexes,liver antioxidant indexes and metabo-lism related gene expression of lambs.Sixty-three Hulunbuir lambs with similar body weight and good physical condition were selected as experimental animals and were randomly divided into 3 groups with 7 replicates of 3 sheep per replicate.The proportions of replacing soybean meal with milk thistle meal in the three diets were 0(group Ⅰ),50％(group Ⅱ)and 100％(group Ⅲ),respectively.The experimental period was a total of 90 days,with a pre feeding period of 27 days and a trial period of 63 days.Weighed and recorded the experi-mental lambs every month during the experimental period,and recorded the feed intake every day.After the experiment,nine lambs were randomly selected from each group for blood collection,and three lambs were slaughtered to collect liver tissue samples for subsequent testing.The results showed as follows:1)the growth performance of lambs in groups Ⅰ,Ⅱ and Ⅲ had no significant difference(P＞0.05).2)There were no sig-nificant differences in serum metabolic indexes among groups Ⅰ,Ⅱ and Ⅲ(P＞0.05);the plasma interleu-kin-1β(IL-1β),tumor necrosis factor-a(TNF-α),transforming growth factor-β(TGF-β),hepatocyte growth factor(HGF),vascular endothelial cell growth factor(VEGF)contents of lambs in group Ⅲ were significantly higher than those in group Ⅰ(P ≤ 0.05),but the insulin-like growth factor-1(IGF-1)and inter-leukin-2(IL-2)contents were not significantly different among groups(P＞0.05).3)There were no signifi-cant differences in liver antioxidant indexes among groups(P＞0.05).Among them,the antioxidant capacity(T-AOC),the activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in liver in group Ⅲ were higher than those in group Ⅰ(P＞0.05),and the malondialdehyde(MDA)content in groupⅢ was lower than that in group Ⅰ(P＞0.05).4)The relative expression levels of liver lipid metabolism relat-ed genes acetyl-CoA acyltransferase 1(ACAA1),aldehyde dehydrogenase 3 family member A2(ALDH3A2)and fatty acid synthase(FASN)in group Ⅲ were higher than those in group Ⅰ,but the differences were not significant(P＞0.05);the relative expression level of liver glucose metabolism related gene fructose-bisphos-phatase 1(FBP1)in group Ⅲ was significantly higher than that in group Ⅰ(P≤0.05),and the relative ex-pression levels of phosphoenolpyruvate carboxykinase 2(PCK2)and glucose-6-phosphatase catalytic subunit(G6PC)were higher than those in group Ⅰ,but the differences were not significant(P＞0.05);the relative expression levels of liver tricarboxylic acid cycle pathway related genes succinate dehydrogenase complex sub-unit B(SDHB)and succinate dehydrogenase complex subunit C(SDHC)in group Ⅲ were significantly higher than those in group Ⅰ(P＜0.05),and the relative expression level of isocitrate dehydrogenase 3β subunit(IDH3B)was higher than those in group Ⅰ,but the difference was not significant(P＞0.05).Therefore,u-sing milk thistle meal to replace soybean meal in the diet does not have a negative impact on growth perform-ance and liver metabolism of lambs.Simultaneously,it can enhance the contents of immune cytokines in blood,and helps improving disease resistance.Moreover,the milk thistle meal can improve liver antioxidant capacity,upregulate liver metabolic gene expression,and accelerate energy metabolism cycle of lambs,so the milk thistle meal has a positive effect on production.[Chinese Journal of Animal Nutrition,2024,36(4):2524-2540]
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维普
