Study on Plasma Metabolomics before and after Short Distance Race of Speed Yili Horses
This study aimed to screen the plasma differential metabolites before and after short distance race of Yili horses by non-targeted metabolomics technology.Six Yili horses with the best performance of 2 000 m in Xinjiang Speed Racing Open were selected to collect the cervical venous blood before and after the race and prepare plasma.Non-targeted metabolomics detection was performed on the plasma samples based on liquid chromatogre-mass spectrometry(LC-MS),and the differential metabolites before and after short distance races of Yili horses were screened and pathway enrichment analysis was performed.The results showed that compared with the pre-race,the differential metabolites in positive and negative ion modes were screened after the race.Among them,L-leucyl-L-alanine,N-lactophenylalanine,acetylcarnitine,allantoin were significantly higher than those before the race(P＜0.05);creatinine,cortisol,lipoic acid,2-aminoadipic acid,N-acetyl-L-methi-onine,xanthosine were significantly lower than those before the race(P＜0.05).The differential metabolites were significantly enriched in pyrimidine metabolism,phenylalanine metabolism,and alpha-linolenic acid me-tabolism,steroid hormone biosynthesis,bile secretion,aldosterone synthesis and secretion and other pathways.The metabolites of Yili horses change significantly before and after the short distance race,which provide a the-oretical basis for post-race recovery and breeding of excellent horses.[Chinese Journal of Animal Nutrition,2024,36(4):2561-2571]
万方数据
维普
