The purpose of this experiment was to investigate the effects of dietary active yeast on growth per-formance,meat quality and intestinal flora of growing meat rabbits.A total of 360 thirty-five-day-old weaned rabbits were randomly divided into 3 groups with 6 replicates per group and 20 rabbits per replicate.Rabbits in the control group were fed a basal diet(no active yeast added),and others in experimental groups were fed the basal diets supplemented with 500 and 1 000 g/t active yeast,respectively.The experiment lasted for 34 days.The results showed as follows:1)compared with the control group,dietary supplemented with 500 and 1 000 g/t active yeast significantly increased the survival rate of growing meat rabbits(P<0.05),and signifi-cantly decreased the average daily feed intake of growing meat rabbits(P<0.05).2)Compared with the con-trol group,dietary supplemented with 500 g/t active yeast significantly increased the muscle drip loss(P<0.05),while dietary supplemented with 1 000 g/t active yeast significantly decreased the muscle drip loss(P<0.05).3)Compared with the control group,dietary supplemented with 1 000 g/t active yeast significantly in-creased the cecal microbiota Simpson index(P<0.05);at phylum level,dietary supplemented with 1 000 g/t active yeast increased the relative abundance of Firmicutes in cecum;at genus level,dietary supplemented with 1 000 g/t active yeast increased the relative abundances of norank_f_Lachnospiraceae,norank_f_Muribacu-laceae,Lachnospiraceae_NK4A136_group and norank_f_norank_o_Clostridia_vadinBB60_group in cecum.4)The analysis of inter group species differences at the genus level showed that compared with the control group,dietary supplemented with 500 and 1 000 g/t active yeast significantly increased the relative abundance of Synergistes in cecum(P<0.05),dietary supplemented with 500 g/t active yeast significantly increased the relative abundance of Anaerostipes in cecum(P<0.05),and dietary supplemented with 1 000 g/t active yeast significantly increased the relative abundance of Bacillus in cecum(P<0.05).In conclusion,dietary supple-mented with 500 and 1 000 g/t active yeast can improve the survival rate of growing meat rabbits,and reduce the average daily feed intake;dietary supplemented with 1 000 g/t active yeast can reduce the muscle drip loss and improve the meat quality of growing meat rabbits;dietary supplemented with 1 000 g/t active yeast can improve the intestinal flora structure of growing meat rabbits,which is conducive to the intestinal health.[Chi-nese Journal of Animal Nutrition,2024,36(4):2603-2611]