Effects of All-Trans Retinoic Acid on Follicular Development,Reproductive Performance and Reproductive Hormone Levels in Mice
The aim of this experiment was to investigate the effects of all-trans retinoic acid(ATRA)on follicular development,reproductive performance and reproductive hormone levels in Kunming mice.Twenty-four female Kunming mice aged 7 weeks were selected.After 5 to 7 days of adaptive feeding,they were ran-domly divided into 4 groups with 6 replicates per group and 1 mouse per replicate.The mice were divided into control group(sterilized distilled water),low-dose group(0.1 mg/kg ATRA),medium-dose group(1.0 mg/kg ATRA)and high-dose group(10.0 mg/kg ATRA).ATRA was given gavage for 14 d.Six Kun-ming mice were sampled from each group,and serum estrogen(E2),progesterone(P4),and follicle stimula-ting hormone(FSH)levels were measured.Their ovaries were isolated and ovarian coefficients were calculat-ed,paraffin sections of ovarian tissue were prepared and the number of the different levels of follicle were counted.Sixty-four proestrus Kunming mice were randomly divided into 4 groups with 16 replicates per group and 1 mouse per replicate.Gavage ATRA continuously for 1 estrous cycle(5 d)and the dosages of ATRA were as above.Six mice were sampled from each group of estrous mice and their serum hormone levels were determined.The remaining 10 mice were cohabited with male mice and weighed once a week during gestation.The litter size,litter birth weight and individual birth weight were counted after the mice delivery.The results showed as follows:1)there was no significant effects of ATRA on body weight and ovarian coefficient of Kunming mice by continuous gavaging ATRA for 14 days compared with the control group(P＞0.05).2)The number of primary follicles in the high-dose group was significantly higher than that in the control group(P＜0.05),and the number of atretic follicles was significantly lower than that in the control group and the low-dose group in the high-dose group(P＜0.05).3)Continuous gavage of ATRA for one oestrus cycle(5 days)did not result in significant changes in body weight during pregnancy in all ATRA groups of Kunming mice(P＞0.05).4)The litter size of Kunming mice in the high-dose group was significantly higher than that in the control group(P＜0.05).The individual birth weight of Kunming mice in the medium and high-dose groups were significantly higher than those in the control group(P＜0.05),and there was no significant difference in the individual birth weight of the experimental group of Kunming mice and the control group(P＞0.05).5)After continuous gavaging ATRA for 5 days,the serum levels of E2,P4 and FSH in the high-dose group were significantly higher than those in the control group(P＜0.05),and E2 level in the high-dose group was signifi-cantly higher than that in the low-dose group and the medium dose group(P＜0.05).Meanwhile,the level of P4 in the medium-dose group was significantly higher than that in the control group and the low-dose group(P＜0.05).In Kunming mice gavaged with ATRA continuously for 14 days,the serum levels of E2,P4 and FSH were significantly higher in the high-dose group than in the control group(P＜0.05),while the level of E2 in the high-dose group was also significantly higher than that in the medium-dose group(P＜0.05).In con-clusion,ATRA can promote the secretion of E2,P4 and FSH reproductive hormones,reduce follicular atresia and promote follicular development of Kunming mice.The addition of 10.0 mg/kg ATRA can significantly im-prove the reproductive performance of Kunming mice.[Chinese Journal of Animal Nutrition,2024,36(4):2623-2632]
万方数据
维普
