Effects of Mixing Ratio,Microbial Preparation and Packing Density on Fermentation Quality and Nutritional Value of Mixed Silage of Corn Straw and Peanut Vine
This experiment was conducted to study the effects of mixing ratio,microbial preparation and pack-ing density on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.This study contained two experiments.Experiment 1 studied the effects of mixing ratio and microbial preparation on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.The corn straw and peanut vine were mixed based on 5 mixing ratios(4∶0,3∶1,2∶2,1∶3 and 0∶4),with 4 groups in each ratio,involving control group(CON group,spraying sterile physiological saline),Lactobacillus plantarum group(LP group,spraying 1×106 CFU/g Lactobacillus plantarum),Enterococcus faecalis group(EF group,spra-ying 1×106 CFU/g Enterococcus faecalis)and Lactobacillus plantarum+Enterococcus faecalis group(LP+EF group,spraying 5×105 CFU/g Lactobacillus plantarum+5×105 CFU/g Enterococcus faecalis).Each group contained 3 replicates,and anaerobic fermentation for 60 days.Experiment 2 studied the effects of packing den-sity and microbial preparation on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.The corn straw and peanut vine were mixed at a ratio of 3∶1,and fermented with different packing densities(300,450 and 600 kg/m3),with 4 groups in each density(same as experiment 1).Each group con-tained 3 replicates,and anaerobic fermentation for 60 days.The results of experiment 1 showed as follows:1)with the increased of peanut vine proportion,the organic matter,neutral detergent fiber and water-soluble car-bohydrate contents were significantly decreased(P＜0.05),while the crude protein content was significantly increased(P＜0.05).Compared with the control group,the neutral detergent fiber content in EF and LP+EF groups was significantly decreased(P＜0.05),and the ammonia nitrogen content in EF group was significantly decreased(P＜0.05).2)The mixing ratio had no significant effects on the lactic acid bacteria and mold num-bers(P＞0.05).Compared with the control group,the lactic acid bacteria number in LP,EF and LP+EF groups was significantly increased(P＜0.05),and the mold number in LP+EF group was significantly de-creased(P＜0.05).3)The zearalenone content in mixing ratio of 2∶2 group was significantly higher than that in other groups(P＜0.05),and the ochratoxin content in mixing ratio of 3∶1 and 2∶2 groups was significantly lower than that in mixing ratio of 4∶0 group(P＜0.05).Compared with the control group,the zearalenone content in LP+EF group was significantly decreased(P＜0.05).4)The pH in mixing ratio of 2:2 group was significantly higher than that in other groups(P＜0.05),the lactic acid content in mixing ratio of 3∶1 group was significantly higher than that in mixing ratio of 2∶2 group(P＜0.05),the acetic acid content in mixing ra-tio of 3∶1 group was significantly lower than that in other groups(P＜0.05),and the butyric acid content in mixing ratio of 3∶1 group was significantly lower than that in mixing ratio of 4∶0 group(P＜0.05).Compared with the control group,the pH in LP+EF group was significantly decreased(P＜0.05),and the lactic acid content was significantly increased(P＜0.05);the acetic acid content in EF group was significantly decreased(P＜0.05).The results of experiment 2 showed as follows:1)the organic matter and crude protein contents in packing density of 600 kg/m3 group were significantly lower than those in packing density of 300 kg/m3 group(P＜0.05),and the water-soluble carbohydrate content was significantly higher than that in other groups(P＜0.05);with the increased of packing density,the ammonia nitrogen content was significantly decreased(P＜0.05).Compared with the control group,the organic content in EF group was significantly increased(P＜0.05),and the crude protein content in LP+EF group was significantly increased(P＜0.05).2)The packing density had no significant effects on the lactic acid bacteria and mold numbers(P＞0.05).Compared with the control group,the lactic acid bacteria number in LP and LP+EF groups was significantly increased(P＜0.05),and the mold number in LP,EF and LP+EF groups was significantly decreased(P＜0.05).3)The zearalenone and fumonin contents in packing density of 450 and 600 kg/m3 groups were significantly higher than those in packing density of 300 kg/m3 group(P＜0.05).Compared with the control group,the zearalenone content in LP+EF group was significantly decreased(P＜0.05).4)The packing density had no significant effect on pH(P＞0.05).The lactic acid content in packing density of 600 kg/m3 group was signifi-cantly higher than that in packing density of 300 kg/m3 group(P＜0.05),and the acetic acid and butyric acid contents in packing density of 300 kg/m3 group were significantly higher than those in other groups(P＜0.05).In conclusion,comprehensive all indexes,the recommended mixing ratio,microbial preparation and packing density of mixed silage of corn straw and peanut vine are 3:1,combination of Lactobacillus plantarum and En-terococcus faecalis,450 to 600 kg/m3,respectively.[Chinese Journal of Animal Nutrition,2024,36(4):2633-2647]
corn strawpeanut vinemixed silagemicrobial preparationpacking density
万方数据
维普
