Screening and Identification of Mycotoxin-Degrading Lactic Acid Bacteria
The aim of this experiment was to obtain lactic acid bacteria which could degrade mycotoxin effi-ciently and had probiotic properties,so as to lay a foundation for their application in feed additives in the fu-ture.By comparing the mycotoxin degrading ability of 169 strains of lactic acid bacteria isolated from the ru-men of Qinghai yak and Tibetan sheep,and comprehensively evaluating the morphology,physiological and bi-ochemical properties,molecular biology,probiotics and security,4 strains were obtained which could simulta-neously degrade 4 mycotoxins in feed materials which were strains C2,E28,C16 and A16.In this study,the highest degradation rates of aflatoxin B1(AFB1),deoxynivalenol(DON),zearalenone(ZEA)and glucosino-late(GLUS)were 70.95％,60.11％,64.00％and 59.18％,respectively,among the 15 strains of lactic acid bacteria obtained from the primary screening;and the degradation rates of AFB1,DON,ZEA and GLUS were 33.53％,60.18％,51.45％and 43.41％by 4 strains obtained from the secondary screening,respectively.The results of 16S rRNA sequencing showed that strains C2 and E28 were Pediococcus acidilactici,strain A16 was Pediococcus pentosaceus,and strain C16 was Enterococcus lactis.These 4 strains of lactic acid bacteria had short fermentation time,strong acid production ability(pH＜4.5),could grow normally in an environment with pH 3.5 and bile salt concentration of 0.1％,and had good self-agglutination and hydrophobicity.Accord-ing to the safety test,all the 4 strains of lactic acid bacteria were non-hemolytic,among which Enterococcus lactis C16 and Laptococcus pentosus A16 were sensitive to erythromycin and penicillin,while Laptococcus lac-tis C2 and E28 were sensitive or moderately sensitive to erythromycin and penicillin,and Laptococcus lactis C2 and E28 had strong antibacterial activity against Staphylococcus aureus,with antibacterial zone diameter was ≥20 mm,and overall there are all excellent candidate strains.[Chinese Journal of Animal Nutrition,2024,36(4):2674-2689]
万方数据
维普
