The aim of this experiment was to establish a method for the determination of gentamicin in feeds by solid phase extraction combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS).The feed samples were extracted with 5％trichloroacetic acid extract,cleaned-up by a mixed cationic solid-phase extraction column(PCX),and determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS),with 2 mmol/L ammonium formate-0.1％formic acid solution(A)-acetonitrile(B)as the mobile phase with gradient elution,and separated on an Agilent SB-Aq column.The linearity of gentamicin content was good in the range of 1.0～100.0 mg/L(R2＞0.995);the limit of detection of the method was 0.03 mg/kg,and the limit of quantification was 0.1 mg/kg;the average recoveries of gentamicin at three spiked levels(1.0,10.0 and 100.0 mg/kg)were 81.5％to 123.6％,and the relative standard deviations＜15％.The method is simple in pretreatment,convenient in operation and suitable for the determination of gen-tamicin in feeds.[Chinese Journal of Animal Nutrition,2024,36(4):2699-2709]
关键词
庆大霉素/液相色谱-串联质谱/固相萃取/饲料
Key words
gentamicin/liquid chromatography-tandem mass spectrometry/solid phase extraction/feed
维普
万方数据