Determination of Gentamicin in Feeds by Solid Phase Extraction Combined with Liquid Chromatography-Tandem Mass Spectrometry
The aim of this experiment was to establish a method for the determination of gentamicin in feeds by solid phase extraction combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS).The feed samples were extracted with 5%trichloroacetic acid extract,cleaned-up by a mixed cationic solid-phase extraction column(PCX),and determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS),with 2 mmol/L ammonium formate-0.1%formic acid solution(A)-acetonitrile(B)as the mobile phase with gradient elution,and separated on an Agilent SB-Aq column.The linearity of gentamicin content was good in the range of 1.0~100.0 mg/L(R2>0.995);the limit of detection of the method was 0.03 mg/kg,and the limit of quantification was 0.1 mg/kg;the average recoveries of gentamicin at three spiked levels(1.0,10.0 and 100.0 mg/kg)were 81.5%to 123.6%,and the relative standard deviations<15%.The method is simple in pretreatment,convenient in operation and suitable for the determination of gen-tamicin in feeds.[Chinese Journal of Animal Nutrition,2024,36(4):2699-2709]
gentamicinliquid chromatography-tandem mass spectrometrysolid phase extractionfeed