Simultaneous Determination of Ethoxyquin and Its Associated Substances in Feeds by High Performance Liquid Chromatography-Tandem Mass Spectrometry
The aim of this study was to establish a method for simultaneous determination of ethoxyquin(EQ),p-phenetidine(PD),phenacetin(PC),ethoxyquin quinone imine(EQI)and ethoxyquin dimer(EQDM)in feeds by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The feed samples were extracted with acetonitrile,purified by BONDESIL-SI multiple impurity adsorption ma-terial,determined by HPLC-MS/MS.The samples were separated with 0.1％formic acid aqueous solution and 0.1％formic acid acetonitrile solution as mobile phase by gradient elution,and separated by Waters BEH C18 column.The results showed that the standard curves of EQ,PD,PC,EQI and EQDM were well linear in the range of 5 to 500 μg/L[coefficient of determination(R2)＞0.995 0].The limits of detection and quantifica-tion for EQ,PD and PC were 3.0 and 10.0 μg/kg,and those for EQI and EQDM were 1.5 and 5.0 μg/kg,respectively.The average recovery rates of EQ,PD,PC,EQI and EQDM at the three supplemental levels(20,50 and 100 μg/kg)ranged from 73.77％to 98.77％,the relative standard deviation(RSD,n=6)with-in the lot was＜10％,and the coefficient of variation between the batches was＜12％.The method is simple and easy to operate,and is suitable for simultaneous determination of EQ and its associated biomass in feeds.[Chi-nese Journal of Animal Nutrition,2024,36(4):2710-2720]
high performance liquid chromatography-tandem mass spectrometryethoxyquinp-phenetidinephenacetinsimultaneous detection
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维普
