Effects of Low-Protein Diet Supplemented with Probiotics on Growth Performance,Fecal Microbiota Composition and Metabolome Feature of Finishing Pigs
This experiment was conducted to study the effects of low-protein diet supplemented with probiotics on the growth performance,fecal microbiota composition and metabolome feature of finishing pigs.Ninety Landrace×Yorkshire crossbred finishing pigs with an average body weight of(80.56±0.45)kg were divided into three groups,they were control group,experimental group Ⅰ and experimental group Ⅱ.Each group had 3 pens(replicates)and each pen had 10 pigs(barrow and sow in half).Pigs in the control group were fed a normal-protein diet containing 12％protein,those in the experimental group Ⅰ were fed a low-protein diet containing 14％protein,and those in the experimental group Ⅱ were fed the low-protein diet supplemented with probiotics(2.0×109 CFU lactic acid bacteria and 2.0×108 CFU Saccharomyces cerevisiae per kg of the low-protein diet).The pre-test period lasted for 5 days,and the formal test period lasted for 42 days.The re-sults showed as follows:1)compared with the control group and experimental group Ⅰ,the average daily gain was significantly increased(P＜0.05),while the feed/gain ratio was significantly decreased in the experi-mental group Ⅱ(P＜0.05).2)Compared with the control group and experimental group Ⅱ,the relative a-bundances of Oscillospirales,Lachnospirales,Lachnospiraceae,Ruminococcaceae,UCG-005,Faecalibacteri-um,Dialister and Fournierella were significantly decreased(P＜0.05),while the relative abundance of Cellu-losilyticum was significantly increased in the experimental group Ⅰ(P＜0.05);compared with the control group,the relative abundances of Lachnospirales,Lactobacillales and Lachnospiraceae were significantly in-creased in experimental group Ⅱ(P＜0.05).3)There were 9,37 and 41 differential metabolites in fecal sam-ples between the experimental group Ⅰ vs.control group,the experimental group Ⅱ vs.control group,and the experimental group Ⅰ vs.experimental group Ⅱ,respectively;the KEGG pathway enrichment analysis for different metabolites between groups obtained 27 enrichment pathways(P＜0.05);compared with the control group and experimental group Ⅰ,the expression level of 5-hydroxyindoleacetic acid was significantly increased(P＜0.05),and the expression level of epinephrine was significantly decreased in fecal samples in the experi-mental group Ⅱ(P＜0.05);the expression level of N-acetyl-L-phenylalanine in fecal samples the in experi-mental group Ⅰ was significantly lower than that in the control group and experimental group Ⅱ(P＜0.05);the expression levels of L-glutamine and D-glucuronic acid in fecal samples in the experimental group Ⅱ were significantly higher than that in the experimental group Ⅰ(P＜0.05).4)Compared with the control group,the contents of ammonia nitrogen,skatole,total branch chain fatty acids and total short chain fatty acids in fe-cal samples were significantly reduced in the experimental group Ⅰ and experimental group Ⅱ(P＜0.05).Compared with the experimental group Ⅰ,the content of acetic acid was significantly decreased(P＜0.05),while the content of butyric acid was significantly increased in the experimental group Ⅱ(P＜0.05).The re-sults indicate that probiotics(lactic acid bacteria and Saccharomyces cerevicae)supplementation to a low-pro-tein diet can improve the structure of gut microbiota and metabolite expression in finishing pigs,reduce the contents of total branch chain fatty acids,ammonia nitrogen and skatole in feces,and increase the average daily gain and feed conversion efficiency of fattening pigs.[Chinese Journal of Animal Nutrition,2024,36(5):2886-2900]
万方数据
维普
