Effects of Compound Bacillus on Performance and Body Health of Laying Hens
This experiment was conducted to investigate the effects of dietary compound Bacillus(Bacillus amyloliquefaciens SC06 and B.subtilis 10)on performance and body health of laying hens.A total of 180 10-week-old Jingbai No.1 laying hens were randomly divided into 2 groups(6 replicates per group and 15 hens per replicate):hens in the control group(CK group)were fed a basal diet,and hens in the experimental group(Bas group)were fed the basal diet supplemented with 5×108 CFU/kg compound Bacillus.The pre-trial period was 1 week and the experimental period was 18 weeks.The results showed as follows:compared with the CK group,1)compound Bacillus increased the average egg weight,eggshell strength and eggshell thick-ness(P＜0.05),however,there were no significant effects on body weight,organ indexes and follicle number of laying hens(P＞0.05);2)the contents of phosphorus and oxytocin in serum were significantly increased by complex Bacillus(P＜0.05),calcium content was increased(P=0.10),and the relative expression level of follicle-stimulating hormone receptor gene in ovary was significantly increased(P＜0.05);3)compound Ba-cillus could significantly increase the total antioxidant capacity(T-AOC)in serum,the activity of glutathionep-eroxidase(GSH-Px)in liver and ovarian,and significantly decrease the malondialdehyde(MDA)content and the gene expression level of Kelch like epichlorohydrin associated protein 1(KEAP1)in ovary(P＜0.05),thus improving the antioxidant capacity of hens;4)compound Bacillus significantly decreased the gene expres-sion levels of interleukin-2(IL-2)and interleukin-8(IL-8)in liver and that of IL-2 in ovary(P＜0.05),while increasing the gene expression level of IL-8 in ovary(P＜0.05).In conclusion,dietary supplementation of 5× 108 CFU/kg compound Bacillus can increase the contents of calcium,phosphorus and follicle-stimulating hor-mone in serum,hormone receptor gene expression levels in ovary and anti-inflammatory and antioxidant capac-ity of the body,so as to improve eggshell quality and production efficiency of laying hens.[Chinese Journal of Animal Nutrition,2024,36(5):3003-3014]
万方数据
维普
