Effects of Dietary Supplementation of Saccharicter-Penin and Lactobacillus plantarum on Immune Function,Intestinal Morphology and Intestinal Flora Structure of Meat Geese
This experiment was conducted to study the effects of dietary supplementation of saccharicter-penin and Lactobacillus plantarum on immune function,intestinal morphology and intestinal flora structure of meat geese,to explore the synergistic relationship and function of saccharicter-penin and Lactobacillus plantarum.Ninety-six 1-day-old Wulong geese with body weight of(0.135±0.020)kg were randomly divided into 4 groups with 4 replicates per group and 6 geese per replicate.The control group(CON group)was fed a basal diet,the saccharicter-penin group(SP group)was fed the basal diet+0.1％saccharicter-penin,the Lactobacil-lus plantarum group(LP group)was fed the basal diet+0.4％Lactobacillus plantarum,and the saccharicter-penin+Lactobacillus plantarum group(SP+LP group)fed the basal diet+0.1％saccharicter-penin+0.4％Lac-tobacillus plantarum.The experiment lasted for 70 days.The results showed as follows:1)compared to the control group,the serum immunoglobulin M(IgM)content of SP group was significantly increased(P＜0.05),the serum immunoglobulin G(IgG)content of LP group was significantly increased(P＜0.05),and the contents IgM and interferon-γ(IFN-γ)in serum of SP+LP group were significantly increased(P＜0.05).2)Compared to the control group,the contents of nterleukin-1β(IL-1β),interleukin-2(IL-2)and tumor necrosis factor-α(TNF-α)in jejunum of SP group,LP group and SP+LP group were significantly decreased(P＜0.05),and the contents of interleukin-4(IL-4)and secretory immunoglobulin A(sIgA)were signifi-cantly increased(P＜0.05).3)Compared to the control group,the jejunal villus height of SP group,LP group and SP+LP group was significantly increased(P＜0.05);the jejunal crypt depth of LP group and SP+LP group was significantly decreased(P＜0.05),and the jejunal villus height/crypt depth was significantly increased(P＜0.05).Compared to the control group,the ileal villus height of LP group and SP+LP group was signifi-cantly increased(P＜0.05);the ileal crypt depth of SP group,LP group and SP+LP group was significantly decreased(P＜0.05),and the ileal villus height/crypt depth was significantly increased(P＜0.05).4)At the phylum level,compared to the control group,the cecal Bacteroidota relative abundance of LP group and SP+LP group was significantly decreased(P＜0.05),and the cecal Campilobacterota relative abundance of SP group,LP group and SP+LP group was significantly decreased(P＜0.05).At the genus level,compared to the control group,the cecal Bacteroides relative abundance of LP group was significantly increased(P＜0.05),the cecal Barnesiella relative abundance of LP group and SP+LP group was significantly increased(P＜0.05),and the cecal Faecalibacterium relative abundance of SP+LP group was significantly increased(P＜0.05).In conclusion,dietary combined supplementation of saccharicter-penin and Lactobacillus plantarum can enhance the immune function of Wulong geese,promote the intestinal morphological development,and improve the structure of intestinal dominant flora.[Chinese Journal of Animal Nutrition,2024,36(5):3024-3034]
万方数据
维普
