Effects of Isosorbide Nitrate on Methane Production,Rumen Fermentation Parameters and Nutrient Degradation Rates of Dairy Cows in Vitro Simulated Rumen Fermentation
The aim of this experiment was to investigate the effects of different levels of isosorbide dinitrate(ISDN)on methane production,rumen fermentation parameters and nutrient degradation rates of dairy cows in vitro simulated rumen fermentation.A one-way completely randomized experimental design was used,set up 2 parallel batches,and divided into 5 groups with 5 replicates per group.The ISDN supplemental levels in sub-strates of each group were 0(group A,as control group),0.1％(group B),0.2％(group C),0.4％(group D)and 0.6％(group E),respectively.The substrate in the experiment was total mixed ration(TMR),and the rumen fluid donors were five adult healthy Holstein bulls with similar body condition.At 24 and 48 h of fermentation,the methane production,rumen fermentation parameters and nutrient degradation rates were measured.The results showed as follows:1)at 24 and 48 h of fermentation,the gas production methane production and methane proportion of group D and group E were significantly lower than those of group A(P＜0.05).2)At 24 h of fermentation,the rumen pH of group D and group E was significantly low-er than that of group A(P＜0.05).At 24 and 48 h of fermentation,there was no significant difference in ru-men total volatile fatty acid(TVFA)concentration among all groups(P＞0.05),but the rumen acetic acid proportion and acetic acid/propionic acid of group D and group E were significantly lower than those of group A(P＜0.05),and the rumen propionic acid proportion was significantly higher than that of group A(P＜0.05).3)At 24 h of fermentation,the dry matter(DM),neutral detergent fiber(NDF)and acid detergent fiber(ANF)degradation rates of group E were significantly higher than that of group A(P＜0.05),and the NDF degradation rate of group D was significantly lower than that of group A(P＜0.05);at 48 h of fermenta-tion,there were no significant differences in nutrient degradation rates among all groups(P＞0.05).In summa-ry,adding 0.4％ISDN can effectively reduce the methane production during in vitro simulated rumen fermen-tation,and has little impact on nutrient degradation rates.[Chinese Journal of Animal Nutrition,2024,36(5):3061-3069]
in vitro simulated rumen fermentationmethaneisosorbide nitraterumen fermentation parame-tersdairy cows
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