Effects of Dietary Zanthoxylum bungeanum Seeds on Nutrient Apparent Digestibility,Intestinal Development,Immune Function and Digestive Enzyme Activities of Hu Sheep
The aim of this experiment was to study the effects of dietary Zanthoxylum bungeanum seeds on nu-trient apparent digestibility,intestinal development,immune function and digestive enzyme activities of Hu sheep.Twenty-seven healthy 3-month-old Hu sheep male lambs with similar body weight[(25.66±3.03)kg]were selected and randomly divided into 3 groups with 9 sheep in each group.The control group was fed a bas-al diet,and the test groups Ⅰ and Ⅱ were substituted with 3％and 6％Zanthoxylum bungeanum seeds in equal amounts for part of the roughage in the basal diet,respectively.The pre-test period was 7 days,and the formal test period was 90 days.The results showed as follows:1)the crude protein(CP)apparent digestibility of test group Ⅱ was significantly lower than that of the control group(P＜0.05).The apparent digestibility of ether extract(EE)and calcium of test groups Ⅰ and Ⅱ was significantly higher than those of the control group(P＜0.05),and the EE apparent digestibility of test group Ⅱ was significantly higher than that of test group Ⅰ(P＜0.05).2)The duodenal villus height/crypt depth of test group Ⅰ was significantly higher than that of the control group(P＜0.05),and the jejunal villus height and villus height/crypt depth of test groups Ⅰ and Ⅱwere significantly higher than those of the control group(P＜0.05).3)The contents of immunoglobulin G(IgG),complement 3(C3)and complement 4(C4)in serum of test group Ⅱ were significantly higher than those of the control group(P＜0.05),and the serum lysozyme(LZM)activity of test groups Ⅰ and Ⅱ was significantly higher than that of the control group(P＜0.05).4)The relative expression levels of Toll-like re-ceptor 2(TLR2)mRNA in duodenum and ileum of test group Ⅰ were significantly higher than those of control group and test group Ⅱ(P＜0.05),and the relative expression level of TLR2 mRNA in jejunum of test groupⅠ was significantly higher than that of the control group(P＜0.05).The relative expression levels of TLR2 protein in duodenum,jejunum,and ileum of test group Ⅰ were significantly higher than that of control and test group Ⅱ(P＜0.05).5)The duodenal amylase activity of test groups Ⅰ and Ⅱ was significantly higher than that of the control group(P＜0.05),and the ileal amylase activity of test groups Ⅰ and Ⅱ was significantly higher than of the control group(P＜0.05).In conclusion,the addition of Zanthoxylum bungeanum seeds in di-et can promote the intestinal development of Hu sheep and improve the intestinal immune function,and the ad-dition level of 3％has the best effect.[Chinese Journal of Animal Nutrition,2024,36(5):3131-3142]
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