动物营养学报2024,Vol.36Issue(5) :3170-3182.DOI:10.12418/CJAN2024.272

开食料中添加纤维素酶对羔羊生长性能、瘤胃发酵和血清生化指标的影响

Effects of Adding Cellulase to Starter Diets on Growth Performance,Rumen Fermentation and Serum Biochemical Indices of Lambs

尹鹏飞 王继卿 郝志云 霍应栋 王元生 赵海碧 王尚龙 唐德富 任春燕
动物营养学报2024,Vol.36Issue(5) :3170-3182.DOI:10.12418/CJAN2024.272

开食料中添加纤维素酶对羔羊生长性能、瘤胃发酵和血清生化指标的影响

Effects of Adding Cellulase to Starter Diets on Growth Performance,Rumen Fermentation and Serum Biochemical Indices of Lambs

尹鹏飞 1王继卿 1郝志云 1霍应栋 2王元生 3赵海碧 1王尚龙 1唐德富 1任春燕1
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作者信息

  • 1. 甘肃农业大学动物科学技术学院,兰州 730070
  • 2. 会宁县郭城驿镇畜牧兽医站,会宁 730726
  • 3. 兰州市副食品基地建设中心,兰州 730070
  • 折叠

摘要

本试验旨在研究开食料中添加纤维素酶对羔羊断奶前和断奶后生长性能、养分消化率、瘤胃发酵及血清生化指标的影响.试验选择初生重相近的24只10日龄湖羊公羔,随机分为2组,每组12只.对照组饲喂未添加纤维素酶的开食料,试验组饲喂添加0.1%纤维素酶的开食料.试验期111 d.羔羊1~9日龄随母羊采食哺乳,10日龄与母羊分开,单栏饲养,并开始饲喂代乳粉和开食料,61日龄断代乳粉.结果表明:1)断奶前和断奶后,开食料中添加纤维素酶显著降低羔羊平均日采食量(ADFI)(P<0.05);断奶前,与对照组相比,试验组羔羊平均日增重(ADG)显著提高(P<0.05),料重比(F/G)显著降低(P<0.05);断奶后,与对照组相比,试验组羔羊ADG显著降低(P<0.05).2)开食料中添加纤维素酶显著提高羔羊断奶前中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)消化率(P<0.05),并显著提高断奶后干物质(DM)和有机物(OM)消化率(P<0.05).3)断奶前和断奶后,开食料中添加纤维素酶显著降低羔羊瘤胃氨态氮和异戊酸含量(P<0.05);断奶前,与对照组相比,试验组羔羊瘤胃总挥发性脂肪酸、乙酸、丙酸和丁酸含量显著提高(P<0.05),瘤胃pH显著降低(P<0.05);断奶后,与对照组相比,试验组瘤胃总挥发性脂肪酸、乙酸、丙酸、异丁酸和戊酸均显著降低(P<0.05).4)断奶前,开食料中添加纤维素酶显著提高羔羊血清总蛋白(TP)、白蛋白(ALB)、葡萄糖(GLU)含量及谷丙转氨酶(ALT)活性(P<0.05),显著降低血清球蛋白(GLB)和尿素(UREA)含量(P<0.05);断奶后,开食料中添加纤维素酶显著提高血清GLB和总胆固醇(TC)提高(P<0.05),显著降低血清ALB、UREA和GLU含量(P<0.05).综上所述,开食料中添加纤维素酶能够提高羔羊断奶前和断奶后对饲粮养分的消化率,改善断奶前瘤胃发酵,提高其断奶前生长性能.

Abstract

This experiment was conducted to investigate the effects of adding cellulase to starter diets on growth performance,nutrient digestibility,rumen fermentation and serum biochemical indices of lambs before and af-ter weaning.Twenty-four male Hu sheep of 10-day-old with similar birth weight were randomly divided into 2 groups with 12 lambs in each group.Lambs in the control group were fed the starter diet without cellulase,and those in experimental group were fed the starter diet supplemented with 0.1%cellulase.The experiment lasted for 111 days.Lambs were fed and suckled with ewe from 1 to 9 days of age,separated from ewe at 10 days of age,raised in a single pen,and started to feed milk replacer powder and starter diet,then stopped feeding milk replacer powder at 61 days of age.The results showed as follows:1)before and after weaning,adding cellu-lase to starter diets significantly decreased the average daily feed intake(ADFI)of lambs(P<0.05);before weaning,compared with the control group,the average daily gain(ADG)of lambs in experimental group was significantly increased(P<0.05),and the ratio of feed to gain(F/G)was significantly decreased(P<0.05);after weaning,ADG in experimental group was significantly decreased compared with the control group(P<0.05).2)Adding cellulase to starter diets significantly increased the digestibilities of neutral detergent fiber(NDF)and acid detergent fiber(ADF)of lambs before weaning(P<0.05),and significantly increased the digestibility of dry matter(DM)and organic matter(OM)after weaning(P<0.05).3)Before and after weaning,adding cellulase to starter diets significantly decreased the rumen contents of ammonia nitrogen and isovaleric acid of lambs(P<0.05);before weaning,compared with the control group,the rumen contents of total volatile fatty acids,acetic acid,propionic acid and butyric acid of lambs in experimental group were sig-nificantly increased(P<0.05),and the rumen pH was significantly decreased(P<0.05);after weaning,compared with the control group,the rumen contents of total volatile fatty acids,acetic acid,propionic acid,isobutyric acid and valerate acid in experimental group were significantly decreased(P<0.05).4)Before wea-ning,adding cellulase to starter diets significantly increased the contents of total protein(TP),albumin(ALB)and glucose(GLU)and the alanine aminotransferase(ALT)activity of in serum of lambs(P<0.05),and significantly decreased the contents of globulin(GLB)and urea(UREA)in serum(P<0.05);after weaning,adding cellulase to starter diets significantly increased the contents of GLB and total cholesterol(TC)in serum(P<0.05),and significantly decreased the contents of ALB,UREA and GLU in serum(P<0.05).In conclusion,adding cellulase to starter diets can improve the digestibilities of dietary nutrients before and after weaning,improve the rumen fermentation before weaning and improve growth performance of lambs before weaning.[Chinese Journal of Animal Nutrition,2024,36(5):3170-3182]

关键词

羔羊/纤维素酶/生长性能/养分消化率/瘤胃发酵/血清生化指标

Key words

lambs/cellulase/growth performance/nutrient digestibility/rumen fermentation/serum bio-chemical indices

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基金项目

公招博士科研启动金项目(GAU-KYQD-2018-28)

国家自然科学基金(32160800)

兰州市科技计划(2022-2-91)

兰州市科技计划(2021-1-162)

出版年

2024
动物营养学报
中国畜牧兽医学会

动物营养学报

CSTPCD北大核心
影响因子:1.297
ISSN:1006-267X
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