Effects of Dietary Alanyl-Glutamine Supplementation on Growth Performance,Serum Biochemical Indices,Liver Antioxidant Capacity,Intestinal Digestive Enzyme Activities and Morphological Structure of Paramisgurnus dabyranus
This experiment was conducted to investigate the effects of alanyl-glutamine(Ala-Gln)on growth performance,serum biochemical indices,liver antioxidant capacity,intestinal digestive enzyme activities and morphological structure of Paramisgurnus dabyranus.A total of 1 440 seedlings with an initial body weight of(4.07±0.01)g were randomly divided into 6 groups with 4 replicates per group,and with 60 fish in each rep-licate.Diets containing 0(control),0.25％,0.50％,0.75％,1.00％and 1.25％Ala-Gln were prepared,re-spectively.The animals were fed for 8 weeks.Results showed that the weight gain rate(WGR),specific growth rate(SGR)and protein efficiency ratio of Paramisgurnus dabyranus which were significantly increased in 0.25％to 1.00％groups compared with control group(P＜0.05),and reached the maximum value in 0.75％group.The hepatosomatic ratio and viscerosomatic ratio of Paramisgurnus dabyranus were significantly increased in 0.75％group compared with control group(P＜0.05).Serum total antioxidant capacity(T-AOC)and immunoglobulin M(IgM)content in 0.75％group were significantly higher than those in control group(P＜0.05);dietary supplementation of 0.25％to 1.00％Ala-Gln significantly increased liver superoxide dis-mutase(SOD)activity(P＜0.05),dietary supplementation of 0.50％to 1.00％Ala-Gln significantly in-creased catalase(CAT)activity(P＜0.05),supplementation with 0.50％Ala-Gln significantly increased glu-tathione(GSH)content(P＜0.05),supplementation with 0.75％and 1.00％Ala-Gln significantly increased glutathione peroxidase(GPX)activity(P＜0.05).The activities of SOD and GPX were the highest in 1.00％group,and the activity of CAT was the highest in 0.75％group.The GSH content was the highest in 0.50％group(P＜0.05).The MDA content in liver of all groups was significantly decreased after adding Ala-Gln(P＜0.05),and 0.75％group was the lowest.In intestinal digestive enzymes,trypsin activity in 0.75％and 1.00％groups was significantly increased compared with control group(P＜0.05),and reached the maximum in 0.75％group.Compared with the control group,dietary supplementation of 0.75％to 1.25％Ala-Gln signif-icantly increased intestinal villus height(P＜0.05),dietary supplementation of 0.25％to 1.25％Ala-Gln signif-icantly increased villus width(P＜0.05),and both reached the maximum when the supplemental level was 0.75％.In conclusion,the supplements of Ala-Gln in diets can promote the growth of Paramisgurnus dabyra-nus,improve antioxidant capacity and immune capacity,improve intestinal morphology and promote digestion and absorption,among which 0.75％supplementation has the best effect.[Chinese Journal of Animal Nutri-tion,2024,36(5):3219-3230]
alanyl-glutamineParamisgurnus dabyranusgrowth performanceantioxidant capacityintesti-nal morphology and structure
万方数据
维普
