A Measurement Method of Seven Free Monosaccharides in Corn Stalks by High-Performance Liquid Chromatography
The objective of this study was to establish an efficient high-performance liquid chromatography method for the determination of seven free monosaccharides[D(+)-glucose,L-arabinose,D(+)-xylose,L-ribose,D(-)-ribose,D(+)-galactose and D(+)-mannose]in corn stalks.Corn stalks were dried at 60 ℃ and ground into powder before being extracted with water at 98 ℃ for one hour,after cooling and centrifuga-tion to obtain the supernatant;it was derivatized using 1-phenyl-3-methyl-5-pyrazolone(PMP),filtered through a 0.22 μm membrane filter and analyzed by high-performance liquid chromatography using an Agilent Zorbax SB-C18 column,and the seven components were separated within 56 min.The results showed that the intra-day relative standard deviations of retention time and peak area for D(+)-glucose,L-arabinose,D(+)-xylose,L-ribose,D(-)-ribose,D(+)-galactose and D(+)-mannose were less than 1.0％and 5.0％,re-spectively;and inter-day relative standard deviations were less than 1.0％and 7.5％,respectively;the linear ranges were 0.31 to 2 000 μg/mL,0.31 to 200 μg/mL,0.50 to 200 μg/mL,0.75 to 200 μg/mL,0.31 to 200 μg/mL,0.75 to 200 μg/mL and 0.31 to 200 μg/mL,with good linearity,the correlation coefficients were all above 0.999;the detection limits were 0.10 to 0.25 μg/mL,and the spiked recoveries were 79.9％to 114.8％.In conclusion,the method established in this study is not only simple to operate,but can also accu-rately quantify seven free monosaccharides in corn straw,this method provides technical support for studying sugar content in corn stalks as well as their digestion and absorption in animals.[Chinese Journal of Animal Nutrition,2024,36(5):3274-3283]
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