Therapeutic Effects of Sodium Valerate on Lipopolysaccharide-Induced Acute Enteritis Injury in Mice
The purpose of this study was to investigate the therapeutic effects of sodium valerate on lipopolysac-charide(LPS)-induced acute enteritis injury in mice.Twenty-four 6-week-old male C57BL/6J mice with simi-lar body weight were randomly divided into 3 groups as control group(CON group),lipopolysaccharide group(LPS group)and sodium valerate group(SV group),with 8 mice in each group.Mice in LPS group and SV group were intraperitoneally injected with LPS 2.5 mg/kg BW,while mice in CON group were intraperitoneal-ly injected with the same volume of normal saline.After 24 h,mice in CON group and LPS group were fed di-et and water freely,while mice in SV group were fed 0.2 mL 100 mg/kg BW sodium valerate every day for 7 consecutive days.The results show that compared with CON group,the colon length in mice in LPS group was significantly decreased(P＜0.05),the serum interleukin-1 β(IL-1β)content and spleen index were signifi-cantly increased(P＜0.05),the contents of acetic acid,propionic acid,butyric acid,valeric acid and total short-chain fatty acid in colon were significantly decreased(P＜0.05),the Bacteroidota relative abundance in colon was significantly increased(P＜0.05),and the Lachnospiraceae_NK4A136_group relative abundance in colon was significantly decreased(P＜0.05).Compared with LPS group,the colon length in mice in SV group was significantly increased(P＜0.05),the serum contents of IL-1β and tumor necrosis factor-α(TNF-α)as well as spleen index were significantly decreased(P＜0.05),the contents of acetic acid,valeric acid and total short-chain fatty acid in colon were significantly increased(P＜0.05),the Akkermansia relative abundance in colon was significantly decreased(P＜0.05),and the Desulfovibrionaceae_unclassified relative abundance in colon was significantly increased(P＜0.05).Compared with CON group,the Shannon index and Simpson in-dex of colonic microflora in mice in SV group were significantly increased(P＜0.05),the Akkermansia rela-tive abundance in colon was significantly decreased(P＜0.05),and the Parabacteroides relative abundance in colon was significantly increased(P＜0.05).In conclusion,the sodium valerate can reduce the serum inflam-matory factor contents in mice with LPS-induced acute enteritis injury,increase the SCFA content in colon,and improve the microbiota balance in colon,so as to exert its therapeutic effect on acute enteritis.[Chinese Journal of Animal Nutrition,2024,36(5):3284-3295]
万方数据
维普
