Effects of Lactic Acid Bacteria and Cellulase on Quality and in Vitro Rumen Fermentation Characteristics of Mixed Silage of Chinese Water Chestnut Residue and Corn Gluten Meal
The aim of this study was to evaluate the effects of different additives on the fermentation quality,chemical composition,microorganism number and in vitro rumen fermentation characteristics of mixed silage of Chinese water chestnut residue(CWCRS)and corn protein meal(CGM).The CWCRS and CGM were mixed evenly according to the ratio of 76∶24 as silage raw materials,and the following treatments were carried out during the silage process:1)did not add lactic acid bacteria and cellulase(CON group);2)added 1 × 106 CFU/g lactic acid bacteria(LAB group);3)added 50 U/g cellulase(CE50 group);4)added 100 U/g cellulase(CE100 group);5)added 1×106 CFU/g lactic acid bacteria+50 U/g cellulase(LAB+CE50 group);6)added 1×106 CFU/g lactic acid bacteria+100 U/g cellulase(LAB+CE100 group).Each group had 3 repli-cates and each replicate had 2 parallel replicates.After mixed silage for 60 days,the silage bags were opened and sampled for analysis.The results showed as follows:in terms of chemical composition,addition of lactic acid bacteria combined with cellulase in silage could significantly reduce the neutral detergent fiber(NDF),acid detergent fiber(ADF),acid detergent lignin(ADL),cellulose and hemicellulose contents of mixed si-lage compared with the CON group(P＜0.05),and significantly increase the crude protein(CP)content(P＜0.05),and the highest CP content was found in LAB+CE100 group.In terms of fermentation quality,the pH,ammoniacal nitrogen(NH3-N)content of mixed silage in LAB,LAB+CE50 and LAB+CE100 groups were sig-nificantly decreased compared with the CON group(P＜0.05),meanwhile,the lactic acid and acetic acid con-tents of mixed silage showed a significant increase compared with the CON group(P＜0.05),and highest lac-tic acid and acetic acid contents were found in the LAB+CE100 group.Addition of lactic acid bacteria alone or combined with cellulase could significantly increase the number of lactic acid bacteria in mixed silage(P＜0.05),and reduce the number of undesirable bacteria or inhibit the proliferation of undesirable bacteria.In terms of in vitro gas production,with the continuous prolongation of fermentation time,the gas production was elevated in all groups,with LAB+CE100 group having the highest gas production rate and gas production at 48 h.Regarding in vitro rumen fermentation parameters,the pH of fermentation broth of each group was be-tween 6.56 to 6.84,and showed a negative correlation trend with fermentation time.The contents of total vola-tile fatty acids,acetic acid,propionic acid and butyric acid in fermentation broth in all additive groups were significantly increased compared with CON group(P＜0.05),and the ratio of acetic acid/propionic acid in ad-ditive groups with lactic acid bacteria showed a significant decrease compared with the CON group(P＜0.05).It can be seen that the addition of lactic acid bacteria combined with cellulase can improve the mixed silage quality and in vitro rumen fermentation characteristics,and the best effect is obtained when adding 1 × 108 CFU/g lactic acid bacteria+100 U/g cellulase.[Chinese Journal of Animal Nutrition,2024,36(5):3340-3351]
lactic acid bacteriacellulaseChinese water chestnut residuecorn gluten mealmixed silage
万方数据
维普
