Effects of Different Lactic Acid Bacteria Additives on Silage Quality of Natural Pasture Grass Silage
In this study,the effects of different lactic acid bacteria additives on nutritional quality,fermentation quality and microbial diversity of natural pasture grass silage were evaluated to improve the silage quality of natural pasture grass silage.The natural pasture grass of meadow steppe was used as ensiling raw material,with five treatments of control(CK),and additions of Limosilactobacillus fermentum(LF),Latilactobacillus gra-minis(LG),Latilactobacillus plantarum(LP)and Lentilactobacillus buchneri(LB)treatments were set up with three replicates in each treatment,and the indicators related to nutritional quality,fermentation quality and bacterial community structure of silage samples were determined after 60 days of ensiling.The results showed that four lactic acid bacteria additives improved the fermentation quality of natural pasture grass silage and re-duced the loss of nutritional components to different degrees compared with the CK treatment.The LG treat-ment had the lowest pH and the highest lactic acid content,the acetic acid and ammoniacal nitrogen contents of the LF and LB treatments were significantly higher than of the other treatments(P＜0.05),and butyric acid was not detected in all treatments.LG treatment had the highest content of water-soluble carbohydrate,and neutral detergent fiber content of the LF and LG treatments was significantly lower than that of the other treat-ments(P＜0.05).Based on high-throughput sequencing,it was found that the abundances of Lentilactobacillus buchneri,Lactiplantibacillus plantarum and Limosilactobacillus fermentum were higher in the lactic acid bacte-ria additive treatments,while the abundances of Levilactobacillus brevis and Leuconostoc mesenteroides was higher in the CK treatment,and there was also a certain proportion of undesirable bacteria.In summary,com-bined with the comprehensive evaluation of the indicators of nutritional quality,fermentation quality and bacte-rial diversity,the silage quality of natural pasture grass silage is the best when Latilactobacillus graminis is add-ed.[Chinese Journal of Animal Nutrition,2024,36(5):3352-3362]
万方数据
维普
