Effects of Different Proportions of Whole Rape Silage Replacing Whole Corn Silage on Simulated Rumen Fermentation Parameters in Vitro
This study was conducted to investigate the effects of different proportions of whole rape silage re-placing whole corn silage on total gas,hydrogen,methane and volatile fatty acid production of simulated ru-men fermentation in vitro.A single-factor experimental design was employed,with whole rape silage replacing whole corn silage at proportion of 0(control),25％,50％,75％,75％and 100％,respectively.Three Xiangdong black goats with permanent rumen fistula were selected as rumen fluid donors for a 72 h simulated rumen fermentation experiment in vitro.The results showed as follows:1)the organic matter degradation rate,gas production per gram of substrate,gas production per gram of degraded substrate,potential maximum gas production,gas production rate and initial substrate degradation rate,and the gas production per gram of sub-strate,gas production per gram of degraded substrate,potential maximum gas production and gas production rate of methane,as well as the hydrogen consumption rate,total volatile fatty acid content and propionic acid ratio were linearly decreased with the increase of whole rape silage proportion(P＜0.05).The gas production per gram of substrate,gas production per gram of degraded substrate,potential maximum gas production and gas production rate of hydrogen,the pH,ammonia nitrogen content,acetic acid,isobutyric acid,valeric acid,isovaleric acid ratios and acetic acid to propionic acid ratio were all linearly increased with the increase of whole rape silage proportion(P＜0.05).2)Compared with the control group,when the whole rape silage proportion ≥ 50％,the organic matter degradation rate,gas production per gram of substrate,gas production per gram of degraded substrate and gas production rate,and the gas production per gram of substrate,potential maximum gas production and gas production rate of methane as well as propionic acid ratio were significantly decreased(P＜0.05);the gas production per gram of degraded substrate,potential maximum gas production and gas production rate of hydrogen,the pH,ammonia nitrogen content,acetic acid,isobutyric acid,valeric acid,isovaleric acid ratios and acetic acid to propionic acid ratio were significantly increased(P＜0.05).In conclusion,although the whole rape silage replacing corn silage can inhibit methane production of simulated ru-men fermentation in vitro,when the proportion is 50％,the organic matter degradation rate significantly de-creases,indicating that the proportion of whole rape silage replacing whole corn silage should not exceed 50％.[Chinese Journal of Animal Nutrition,2024,36(5):3363-3372]
万方数据
维普
