摘要
本试验测定了生长猪的氨基酸表观回肠消化率(AID)和标准回肠消化率(SID),并基于小麦麸有效化学成分含量构建了氨基酸的SID预测模型.选取22头"杜×(长×大)"生长猪[体重(34.3±4.3)kg],在其回肠末端安装"T"型瘘管,按照2个"11×3"的不完全拉丁方设计,分别饲喂1个无氮饲粮和10个小麦麸饲粮,每个处理共计6个重复,试验共3个连续周期,每期7 d,包括5 d的预饲期和2 d回肠食糜收集期.结果显示:饲喂基础下,10个小麦麸的赖氨酸(Lys)、蛋氨酸(Met)、苏氨酸(Thr)和色氨酸(Trp)含量的平均值分别为0.93%(0.76%~1.19%)、0.31%(0.27%~0.40%)、0.53%(0.46%~0.62%)和 0.17%(0.13%~0.23%),变异系数(CV)分别为 19.19%、14.19%、11.70%和 8.32%;生长猪对 10 个小麦麸 AIDLys、AIDMet、AIDThr、AIDTrp 的平均 值分别 为 67.03%(56.09%~75.72%)、50.96%(42.93%~56.68%)、45.91%(38.26%~52.29%)和 76.55%(73.30%~81.65%),SIDLys、SIDMet、SIDThr、SIDTrp的平均值分别为82.36%(73.21%~90.05%)、76.68%(73.39%~81.55%)、60.99%(53.14%~65.67%)和 88.79%(86.21%~92.03%).SIDLys、SIDMet最佳预测方程分别为:SIDLys=128.691+312.567×钙(Ca)-14.555×粗灰分(Ash)(R2=0.687);SIDMet=86.306-2.201 ×粗脂肪(EE)(R2=0.490).由上可知,本试验中的10个小麦麸氨基酸组成差异较大,EE、Ca和Ash含量可作为生长猪小麦麸SIDLys和SIDMet的关键预测因子.
Abstract
In this experiment,the apparent ileal digestibility(AID)and standardized ileal digestibility(SID)of amino acids of wheat bran for growing pigs were determined,and predictive models for SID of amino acids based on the contents of effective chemical components in wheat bran were established.Twenty-two"Duroc×(Landrace×Large)"growing pigs[body weight of(34.3±4.3)kg]were selected and fitted with T-shaped fistulas at the end of their ileum.According to two incomplete Latin square designs of"11×3",the pigs in each treatment were fed a nitrogen-free diet and 10 wheat bran diets,respectively,for 3 consecutive cycles,each period lasted for 7 days.It included 5 days pre-feeding period and 2 days ileal digesta collection period.The results showed that,on as-fed basis,the average contents of lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp)in ten wheat bran were 0.93%(0.76%to 1.19%),0.31%(0.27%to 0.40%),0.53%(0.46%to 0.62%)and 0.17%(0.13%to 0.23%),with coefficients of variation(CV)of 19.19%,14.19%,11.70%and 8.32%,respectively.The average AIDLys,AIDMet,AIDThr and AIDTrp of ten wheat bran for growing pigs were 67.03%(56.09%to 75.72%),50.96%(42.93%to 56.68%),45.91%(38.26%to 52.29%)and 76.55%(73.30%to 81.65%),and the average SIDLys,SIDMet,SIDThr and SIDTrp were 82.36%(73.21%to 90.05%),76.68%(73.39%to 81.55%),60.99%(53.14%to 65.67%)and 88.79%(86.21%to 92.03%),respectively.The optimal prediction equations of SIDLys and SIDMet were as follows:SIDLys=128.691+312.567×calcium(Ca)-14.555x crude ash(Ash)(R2=0.687);SIDMet=86.306-2.201× ether extract(EE)(R2=0.490).In summary,the amino acid composition of the 10 wheat bran in this experi-ment has visible difference,and EE,Ca and Ash contents can be used as key predictors of the SIDLys and SIDMet of wheat bran for growing pigs.[Chinese Journal of Animal Nutrition,2024,36(6):3573-3586]
基金项目
国家重点研发计划(2021YFD1300201)
国家重点研发计划(2021YFD1301004)
云南省重大科技专项计划(202202AE090032)
湖南省自然科学基金项目(2022JJ40532)