Study on Different Characteristics of Cecal Enterotypes and Their Association with Cecal Short-Chain Fatty Acid Contents and Slaughter Performance of Muscovy Ducks
The aim of this study was to explore the characteristics of different cecal enterotypes(ETs)and their relationship with cecal short-chain fatty acids(SCFAs)and slaughter performance of Muscovy ducks.Through high-throughput 16S rRNA gene sequencing,cecal enterotypes were identified by the partitioning a-round medoid(PAM)algorithm from 200 Muscovy ducks at 70 days of age.The growth performance,slaughtering performance and cecal SCFAs contents of Muscovy ducks with different ETs were determined.The structure and diversity of cecal microbiota of Muscovy ducks with different ETs at phylum and genus levels and the relationship between the relative abundance of differential genera and SCFAs content in cecum were also an-alyzed.The results showed that two ETs were identified from the cecum of Muscovy ducks:ET1(n=165)re-presented by Parabacteroides and ET2(n=35)represented by Fusobacterium,and the Shannon index of ET1 was significantly higher than that of ET2(P＜0.05).At the genus level,the relative abundances of Bacteroides(29.33％),Ruminococcaceae_uncultured(4.83％)and Parabacteroides(4.68％)were higher in ET1 than those in ET2,while the relative abundances of Bacteroides(20.71％),Fusobacterium(14.56％),and Para-bacteroides(4.29％)were higher in ET2 than those in ET1.Comparative analysis by linear discriminant analy-sis effect size(LEfSe)revealed that the differential genera between different ETs included butyric acid-produ-cing bacteria such as Faecalibacterium,Butyricoccus and[Eubacterium]hallii group and so on.Comparison of the SCFAs contents in the cecum of Muscovy ducks with different ETs revealed that the contents of acetate,propionate and butyrate in the cecum of ET1 were significantly higher than those of ET2(P＜0.05),whereas the contents of isobutyrate,valerate and isovalerate in the cecum did not show any significant difference be-tween ET1 and ET2(P＞0.05).Further Spearman correlation analysis of the relative abundances of differential genera with SCFAs contents revealed significant positive correlations between Faecalibacterium and propionate,butyrate,isobutyrate,valerate and isovalerate(P＜0.05).In addition,comparing the slaughtering performance of Muscovy ducks with different ETs,it was found that the abdominal fat weight and the percentage of abdomi-nal fat of ET2 Muscovy ducks were significantly higher than those of ET1 Muscovy ducks(P＜0.05),while there were no significant differences in the dressed weight,half-eviscerated weight,eviscerated weight dressed percentage,percentage of half-eviscerated yield,percentage of eviscerated yield,breast muscle weight,leg muscle weight,percentage of breast muscle and percentage of leg muscle(P＞0.05).These findings indicate that the difference in cecal microbiota structure between two ETs might result in differences in SCFAs contents in the cecum of Muscovy ducks,which might further influence the slaughter performance.It may provide new insights into the interactions between intestinal microbiota and their hosts.[Chinese Journal of Animal Nutri-tion,2024,36(6):3680-3692]
万方数据
维普
