Effects of Isomaltooligosaccharide Synbiotics on Intestinal Permeability,Fecal Microbiota and Short Chain Fatty Acid Concentrations of Holstein Calves
The aim of this study was to explore the effects of isomaltooligosaccharide synbiotics(composed of isomaltooligosaccharide,Lactobacillus reuteri and Lactobacillus johnsonii)on intestinal permeability,fecal mi-crobiota and short chain fatty acid concentrations of Holstein calves.Selected 40 healthy Holstein calves and di-vide them into 4 groups according to the similar body weight and days of age,and each group had 10 calves.The calves in the 4 groups were supplemented with 0(Con group),2.5(LC group),5.0(MC group)and 10.0 g/(head·d)(HC group)isomaltooligosaccharide synbiotics,respectively.The pre-experimental period lasted for 5 days,and the experimental period lasted for 42 days.The results showed as follows:1)on day 21 of the experiment,the serum endotoxin(ET)content of the LC group was significantly lower than that of Con group(P＜0.05);on day 42 of the experiment,the serum D-lactic acid(D-LA)content of the LG and HG groups was significantly decreased(P＜0.05),and the serum D-LA content and diamine oxidase(DAO)ac-tivity of the MG group were significantly decreased compared with the Con group(P＜0.05).On day 21 of the experiment,the concentration of propionic acid in feces of the MG and HG groups was significantly higher than that of the Con group(P＜0.05);on day 42 of the experiment,the concentration of propionic acid in feces of the MG group was significantly increased,and the concentrations of propionic acid and isovaleric acid in feces of the LG group were significantly increased compared with the Con group(P＜0.05).At the phylum level,the dominant bacteria phyla in all groups were Firmicutes,Bacteroidota,Actinobacteriota.On day 24 of the ex-periment,the fecal Actinobacteriota relative abundance of HG group was significantly higher than that of the Con group(P＜0.05);On day 42 of the experiment,the fecal Firmicutes relative abundance of MG group was significantly higher than that of Con group(P＜0.05).At the genus level,the dominant bacteria genera in all groups were Blautia and Lactobacillus.And the fecal relative abundance of Lactobacillus of the three trial groups was higher than that of the Con group.The correlation analysis fecal microbiota and intestinal permeabil-ity indexes and fecal short chain fatty acids showed that the Lachnoclostridium relative abundance was extreme-ly significant positively correlated with the D-LA content(P＜0.01)and the DAO activity(P＜0.001).The norank_f_Erysipelotrichaceae relative abundance was significant positively correlated with the ET content(P＜0.05).The Holdemanella relative abundance was significant negatively correlated with the DAO activity(P＜0.05).The Lactobacillus relative abundance was significant positively correlated with the concentrations of bu-tyric acid,isobutyric acid and isovaleric acid(P＜0.05).The Parabacteroides relative abundance was signifi-cant negatively correlated with the concentrations of propionic acid,butyric acid,isobutyric acid and isovaleric acid(P＜0.05).The Bacteroides relative abundance was extremely significant positively correlated with the bu-tyric acid concentration(P＜0.001).In summary,isomaltooligosaccharide synbiotics can improve the intestinal permeability of Holstein calves,increase the concentrations of short chain fatty acids in the intestine,change the structure of the intestinal microbiota,and its optimal supplementary dosage is 5 g/(head·d).[Chinese Journal of Animal Nutrition,2024,36(6):3756-3771]
万方数据
维普
