Comparative Analysis of Plasma Metabolome of Kazakh Horses with High and Low Milk Fat Rates
The purpose of this study was to analyze the differences in plasma metabolites of Kazakh horses with different milk fat rates,and to screen out the marker metabolites in plasma of Kazakh horses with high milk fat rate,so as to provide data support for the breeding of Kazakh horses with high milk fat rate.Twelve Kazakh horses with similar age,parity,body condition,and feeding and management conditions during lactation were selected,and 288 milk samples and blood samples were collected from Kazakh horses during days 30 to 105 after delivery.According to the determination results of milk fat rate,six Kazakh horses with high milk fat rate(≥1.84％)were selected,and their blood samples were divided into high milk fat rate group(HF group);six Kazakh horses with low milk fat rate(≤0.90％)were selected,and their blood samples were divided into low milk fat rate group(LF group).Subsequently,plasma metabolites in blood samples were analyzed by non-targeted liquid chromatography-mass spectrometry(LC-MS)metabolomics technique.The results showed that a total of 53 differential metabolites were detected in the plasma of high and low milk fat rate Kazakh horses.The concentrations of 36 substances such as arachidonic acid,D-erythro-sphingosine-l-phosphate,prostaglan-din,5-hydroxyindole-3-acetic acid,thromboxane B2,uric acid and so on in the plasma of the high milk fat rate Kazakh horses were significantly higher than those of the low milk fat rate Kazakh horses(P＜0.05),while the concentrations of 17 substances such as L-pyroglutamic acid,D-fructose-6-phosphate,biotin and D-homocysteine and so on in the plasma of the high milk fat rate Kazakh horses were significantly lower than those of the low milk fat rate Kazakh horses(P＜0.05).These differential metabolites were mainly enriched in metabolic pathways such as arachidonic acid metabolism,linoleic acid metabolism,purine metabolism,galac-tose metabolism,glycine,serine and threonine metabolism,etc.The results of this study provide a theoretical basis for further exploring the synthesis of milk fat and further improving the quality of mare's milk.[Chinese Journal of Animal Nutrition,2024,36(6):3845-3856]
万方数据
维普
