Effects of Tea Tree Oil on Lipopolysaccharide-Induced Injury in Bovine Intestinal Epithelial Cells
This study was conducted to investigate the effects of tea tree oil(TTO)on lipopolysaccharide(LPS)-induced injury in bovine intestinal epithelial cells(BIECs).Jejunum tissues of 3 healthy newborn Chi-nese Holstein calves were isolated to obtain BIECs for culture.Cell inflammation model was established by u-sing different concentrations of LPS(0,1,2 and 4 µg/mL)and TTO(0,0.006 25％,0.012 50％,0.025 00％,0.050 00％and 0.100 00％).Then,the cells in the control group were treated with medium with-out TTO and LPS,those in LPS group were treated with 1 μg/mL LPS for 12 h,and those in LPS+TTO group were treated with 0.012 50％and 0.025 00％TTO for 12 h,and then exposed to LPS for 12 h after wa-ter washing.The results showed as follows:1)compared with the control group(without TTO),the supple-mentation of 0.006 25％,0.012 50％,0.025 00％and 0.050 00％TTO had no significant effects on the cell viability of BIECs(P＞0.05),and the supplementation of TTO significantly increased the transepithelial elec-trical resistance(TEER)(P＜0.05).Compared with the control group,the supplementation of 0.012 50％TTO significantly increased the mRNA relative expression levels of zonula occludens-1(ZO-1),occludin,claudin-1 and claudin-4 in BIECs(P＜0.05),and significantly decreased the tumor necrosis factor-α(TNF-α)mRNA relative expression level(P＜0.05).2)Compared with LPS group,the TEER and mRNA relative ex-pression levels of ZO-1,occludin and claudin-1 in LPS+TTO group were significantly increased(P＜0.05),and the TNF-α mRNA relative expression level was significantly decreased(P＜0.05);the ZO-1 protein rela-tive expression level in LPS+TTO group was significantly increased(P＜0.05).In conclusion,TTO can allevi-ate LPS-induced barrier dysfunction and inflammatory injury in intestinal epithelial cells of calves by up-regula-ting the expression of tight junction protein and down-regulating the expression of inflammatory factor.[Chi-nese Journal of Animal Nutrition,2024,36(6):3942-3951]
tea tree oilbovine intestinal epithelial cellsinflammationtight junction
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