Study on Detoxification Effects of Lactic Acid Bacteria Peptidoglycan on Aflatoxin B1 of Ducklings'Diet
This experiment was conducted to explore the adsorption effect of lactic acid bacteria peptidoglycan on aflatoxin B1(AFB1),so as to provide a theoretical basis for the preparation of lactic acid bacteria pepti-doglycan and the detoxification of AFB1 in diet.The experiment used 4 types of lactic acid bacteria(Lactoba-cillus acidophilus,Streptococcus thermophilus,Lactobacillus reuteri and Lactobacillus plantarum)as raw ma-terials to extract lactic acid bacteria peptidoglycan,the in vitro adsorption rates of 4 types of lactic acid bacteria peptidoglycan on AFB1 were compared by high-performance liquid chromatography,and the Lactobacillus reu-teri peptidoglycan with the best in vitro adsorption effect was selected for animal experiment.A total of 120 healthy Cherry Valley ducks were allocated into 5 groups with 4 replicates per group and 6 ducks per replicate.The control group was fed a basal diet,the AFB1 group was fed the basal diet supplemented with 0.1 mg/kg AFB1,and the experimental groups were fed the AFB1 group diets supplemented with 0.10％(group Ⅰ),0.15％(group Ⅱ)and 0.20％(group Ⅲ)Lactobacillus reuteri peptidoglycan,respectively.The pre-experi-mental period lasted for 3 days,and the experimental period lasted for 21 days.The results showed as follows:1)the species,addition amount and their interaction of lactic acid bacteria peptidoglycan could significantly af-fect the in vitro adsorption rates for AFB1(P＜0.05),and the 10.0 mg/mL Lactobacillus reuteri peptidoglycan had the highest in vitro adsorption rates for AFB1,which reached 75.29％.2)Compared with the control group,the average daily weight gain and average daily feed intake of the AFB1 group were significantly de-creased(P＜0.05),and the feed to gain ratio was significantly increased(P＜0.05);the aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(AKP)activities and malondialdehyde(MDA)content in plasma were significantly increased(P＜0.05),the liver,spleen,thymus and bursa of Fabricius indexes were significantly increased(P＜0.05),and the immunoglobulin G(IgG),immunoglobulin M(IgM),γ-interferon(IFN-γ),interleukin-2(IL-2),interleukin-4(IL-4)contents and superoxide dis-mutase(SOD),glutathione peroxidase(GSH-Px)activities and total antioxidant capacity(T-AOC)in plas-ma were significantly decreased(P＜0.05).3)Compared with the AFB1 group,the average daily weight gain of groups Ⅰ,Ⅱ and Ⅲ were significantly increased(P＜0.05),and the feed to gain ratio was significantly decreased(P＜0.05);the IgG,IFN-γ,IL-2,IL-4 contents and SOD,GSH-Px activities in plasma of groupsⅠ,Ⅱ and Ⅲ were significantly increased(P＜0.05),and the AST,AKP,ALT activities and MDA content in plasma were significantly decreased(P＜0.05);the thymus and bursa of Fabricius indexes of groups Ⅱ andⅢ were significantly decreased(P＜0.05).In conclusion,the lactic acid bacteria peptidoglycan has different in vitro adsorption capacity on AFB1.Dietary supplemented with Lactobacillus reuteri peptidoglycan has a certain recovery effect on the decline in growth performance of ducklings affected by AFB1,and can improve the re-duced immune function and liver toxicity caused by AFB1.[Chinese Journal of Animal Nutrition,2024,36(7):4339-4349]
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