Effects of Mixed Probiotics on Growth Performance,Meat Quality and Intestinal Health of Largemouth Bass(Micropterus salmoides)
The aim of this experiment was to investigate the effects of mixed probiotics(Bacillus coagulans,Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus acidophilus,and viable count ≥ 1.75 × 109 CFU/g)on the growth performance,meat quality and intestinal health of largemouth bass(Micropterus salmoides).A total of 990 largemouth bass with initial body weight of(36.17±1.52)g were randomly divided into 3 groups with 3 replicates in each group and 110 fish in each replicate.Fish in 3 groups were fed the basal diets supplemented with 0(control group,CON group),0.1％(MP-0.1％group)and 1.0％(MP-1.0％group)mixed probiotics,respectively.The experiment lasted for 130 days.The results showed as follows:1)there were no significant differences in growth performance and shape indexes among all groups(P＞0.05).2)Compared with the CON group,the myofiber diameter of MP-0.1％and MP-1.0％groups was significantly de-creased(P＜0.05),and the myofiber density of MP-1.0％group was significantly increased(P＜0.05).3)Compared with the CON group,the muscle hardness,gumminess and chewiness of MP-1.0％group were sig-nificantly increased(P＜0.05).4)Compared with the CON group,the muscle moisture content of MP-1.0％group was significantly decreased(P＜0.05),and the contents of crude protein,flavor amino acids(delicious amino acids and sweet amino acids),polyunsaturated fatty acids,omega-3 fatty acids and omega-6 fatty acids in muscle were significantly increased(P＜0.05);the contents of polyunsaturated fatty acids and omega-3 fatty acids in muscle of MP-0.1％group were significantly increased(P＜0.05),and the muscle monounsaturated fatty acids content was significantly decreased(P＜0.05).5)Compared with the CON group,the contents of geosmin and 2-methyli-soborneol in muscle of MP-0.1％and MP-1.0％groups were significantly decreased(P＜0.05).6)Compared with the CON group,the activities of pepsin and trypsin in intestine of MP-1.0％group were significantly increased(P＜0.05).7)Compared with the CON group,the intestinal villus width of MP-0.1％group was significantly increased(P＜0.05).8)Compared with the CON group,the contents of isovaleric acid and total acid in intestine were significantly increased(P＜0.05).9)The relative abundances of Cetobacterium and Peptostreptococcus in intestine had significantly different among three groups(P＜0.05),and the MP-0.1％and MP-1.0％groups had more similar intestinal flora,which were different from the CON group.In conclusion,dietary mixed probiotics can improve the meat quality and intestinal health of largemouth bass.Under the conditions of this experiment,the dietary mixed probiotics appropriate supplemental level is 1.0％.[Chinese Journal of Animal Nutrition,2024,36(7):4588-4609]
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