Screening Combination of Lactobacillus spp.and Prebiotics and Evaluating Their Impact on Intestinal Barrier Utilizing IPEC-J2 Cell Model
In order to develop green additive products to improve the intestinal health of animals,this study aimed to screen the synbiotic combination of Lactobacillus spp.and prebiotics,and investigate its influence on the integrity of the intestinal barrier.Initially,the fermentation results using API 50 CHL kit were used to iden-tify five strains of Lactobacillus spp.[Lactobacillus plantarum 177(LP),Lactobacillus salivarius KLW001(LSa),Lactobacillus reuteri KLR3004(LRe),Lactobacillus paracaeei KCTC3510(LPa),and Lactobacil-lus rhamnosus KCTC5033(LRh)]corresponding to candidate prebiotics.Then,based on the fermentation a-bility of short-chain fatty acids(SCFA)by the five strains of Lactobacillus spp.and candidate prebiotics[sor-bitol(So),mannitol(Ma),rhamnose(Rh),xylo-oligosaccharide(Xyo),trehalose(Tr),raffinose(Ra),and pullulan(Pu)],synbiotic combinations were selected.Three selected synbiotic combinations(LP+Pu,LSa+Pu,and LRe+Ra)were then applied to lipopolysaccharide(LPS)-induced"leaky gut"cell model.The effect of synbiotics on intestinal barrier function was validated by measuring changes in monolayer cell permeability,trans-epithelial electrical resistance(TEER)values,and the mRNA expression of intestinal tight junction proteins[Claudin-1,Occludin,and zonula occluden-1(ZO-1)].Results showed that the combi-nation of LP+Pu exhibited enhanced fermentation capabilities,resulting in higher production of SCFA,particu-larly an increase in butyric acid content;in the"leaky gut"cell model,the synbiotic consisting of LP+Pu dem-onstrated an upregulation of mRNA relative expression for intestinal tight junction proteins,specifically Clau-din-1 and ZO-1.Furthermore,this synbiotic combination contributed to the preservation of cell epithelial integ-rity.The results of this study confirm that the combination of LP+Pu can serve as a synbiotic formulation,ef-fectively enhancing the production of SCFA and increasing the expression of intestinal tight junction proteins.These findings present a novel avenue for the treatment and prevention of intestinal barrier-related diseases in livestock and poultry.[Chinese Journal of Animal Nutrition,2024,36(7):4693-4705]
Lactobacillus spp.synobioticsshort-chain fatty acidintestinal barrierIPEC-J2 cell line
万方数据
维普
