Effects of Dietary Clostridium butyricum and Sodium Butyrate on Performance,Egg Quality and Intestinal Health of Laying Hens
This experiment was conducted to investigate the effects of dietary Clostridium butyricum and sodi-um butyrate on performance,egg quality and intestinal health of Hy-Line grey laying hens aged from 42 to 50 weeks.A total of 720 healthy Hy-Line grey laying hens of 42-week-old with similar body weight and normal feeding were randomly divided into 4 groups with 6 replicates per group and 30 hens per replicate.Hens in group A(control group)were fed a basal diet;those in group B were fed the basal diet supplemented with 250 g/t Clostridium butyricum(viable bacteria number was 1×1010 CFU/g);those in group C were fed the basal diet supplemented with 500 g/t sodium butyrate(purity≥ 90％);those in group D were fed the basal di-et supplemented with 250 g/t Clostridium butyricum+500 g/t sodium butyrate.The pre-trial period lasted for 5 days and the experimental period lasted for 56 days.The results showed as follows:1)compared with group A,the average daily feed intake and feed to egg ratio of laying hens in group B were significantly decreased(P＜0.05),the feed to egg ratio in group C had a decreasing trend(P＞0.05),and there was no significant difference in performance in group D(P＞0.05).2)Compared with group A,the eggshell ratio in group B was significantly increased(P＜0.05),the egg yolk ratio in group D was significantly increased(P＜0.05),and there was no significant difference in egg quality in group C(P＞0.05).3)Compared with group A,the villus height in duodenum and jejunum of laying hens in groups B,C and D was significantly increased(P＜0.05),and the ratio of villus height to crypt depth in duodenum,jejunum and ileum was significantly in-creased(P＜0.05).4)At the phylum level,the relative abundance of unclassified_k_norank_d_bacteria in ce-cum of laying hens in group B was significantly lower than that in group A(P＜0.05),the relative abundance of Verrucomicrobiota in cecum in group B was significantly higher than that in group C(P＜0.05),and the relative abundance of Fusobacteriota in cecum in group D was significantly higher than that in group C(P＜0.05).At the genus level,compared with group A,the relative abundances of Parabacteroides,Eubacterium_brachy_group and Faecalitalea in cecum of laying hens in group B were significantly increased(P＜0.05),and the relative abundance of unclassified_k_norank_d_bacteria in cecum was significantly decreased(P＜0.05);the relative abundances of Streptococcus and Pediococcus in cecum in group C were significantly de-creased(P＜0.05),and the relative abundance of Ruminiclostridium in cecum was significantly increased(P＜0.05);the relative abundance of Lactococcus in cecum in group D was significantly decreased(P＜0.05).Compared with group B,the relative abundance of Phascolarctobacterium in cecum in group C was significant-ly increased(P＜0.05),and the relative abundance of Eubacterium_brachy_group in cecum was significantly decreased(P＜0.05);the relative abundances of unclassified_f_Prevotellaceae,Desulfovibrio and CHKCI002 in cecum in group D were significantly decreased(P＜0.05).Compared with group C,the relative abundances of Fusobacterium and Parasutterella in cecum in group D were significantly increased(P＜0.05),and the rela-tive abundance of Ruminiclostridium in cecum was significantly decreased(P＜0.05).In conclusion,dietary Clostridium butyricum can improve the performance and increase the eggshell ratio;dietary combination of Clostridium butyricum and sodium butyrate can significantly increase egg yolk ratio;dietary Clostridium butyri-cum and sodium butyrate alone or in combination can improve small intestine morphology,increase the relative abundances of beneficial bacteria in cecum,decrease the relative abundances of harmful bacteria in cecum,and maintain intestinal health of Hy-Line grey laying hens.Under the conditions of this experiment,dietary 250 mg/kg Clostridium butyricum(live bacteria number is 2.5×109 CFU/kg)has a good effect.[Chinese Journal of Animal Nutrition,2024,36(8):4957-4970]
laying hensClostridium butyricumsodium butyrateperformanceegg qualityintestinal health
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