Effects of Eugenol and Glycerol Monolaurate Nanoemulsion on Growth Performance,Antioxidant Capacity and Intestinal Health of Broilers
This experiment aimed to investigate the effects of eugenol(EUG)and glycerol monolaurate(GML)nanoemulsion added in drinking water on the growth performance,antioxidant capacity and intestinal health of broiler chickens.A total of 600 one-day-old white feathered broilers with similar body weight of(39.15±0.11)g were randomly divide into 5 groups with 6 replicates in each group and 20 chickens in each replicate.Broilers in all groups were fed a basal diet,which broilers in the control group were drinking water normally,and broilers in experimental groups were added 0.5,1.0,3.0 and 5.0 mL/L EUG and GML nanoe-mulsion in drinking water,respectively.The experiment lasted for 42 days.The results showed as follows:1)compared with the control group,adding 1.0 and 3.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the body weight at 21 and 42 days of age and daily weight gain during 1 to 21 days of age,22 to 42 days of age and 1 to 42 days of age(P＜0.05),and significantly decreased the feed to gain ratio during 22 to 42 days of age and 1 to 42 days of age(P＜0.05).2)Compared with the control group,adding 1.0 and 3.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the serum total an-tioxidant capacity and glutathione peroxidase activity(P＜0.05),and significantly decreased the serum malond-ialdehyde content(P＜0.05);adding 3.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the serum superoxide dismutase activity(P＜0.05).3)Compared with the control group,adding 1.0,3.0 and 5.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the duodenal and jejunal villus height(P＜0.05),and significantly decreased the duodenal crypt depth(P＜0.05);adding 1.0 and 3.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the duodenal and je-junal villus height/crypt depth(P＜0.05).4)Compared with the control group,adding 3.0 and 5.0 mL/L EUG and GML nanoemulsion to drinking water significantly decreased the gene relative expression levels of tumor necrosis factor-α and interleukin-1β in duodenum and jejunum(P＜0.05),and adding 1.0 and 3.0 mL/L EUG and GML nanoemulsion to drinking water significantly increased the gene relative expression level of interleukin-10 in duodenum and jejunum(P＜0.05).5)There were no significant differences in the cecal microflora ACE,Chao1,Shannon and Simpson indexes among all groups(P＞0.05).Compared with the control group,adding 3.0 mL/L EUG and GML nanoemulsion to drinking water increased the cecal Bacte-roidetes relative abundance,and decreased the Firmicutes relative abundance.In summary,adding EUG and GML nanoemulsion to drinking water can enhance the antioxidant capacity,improve the intestinal morphology,inflammatory response and microbial structure,then increase the growth performance of broilers.In this experi-ment,the suitable added level of EUG and GML nanoemulsion in drinking water is 3.0 mL/L.[Chinese Jour-nal of Animal Nutrition,2024,36(8):5039-5049]
eugenolglycerol monolauratebroilersgrowth performanceantioxidant capacityinflammato-ry responseintestinal health
万方数据
维普
