Effects of Puerarin on Growth Performance,Serum Antioxidant and Immune Indices and Intestinal Health of Broilers Challenged with Lipopolysaccharide
This experiment was conducted to investigate the effects of dietary puerarin(PUE)on growth per-formance,serum antioxidant and immune indices,intestinal morphology and cecal microbiota of broilers chal-lenged with lipopolysaccharide(LPS).A total of 288 yellow-feathered male broilers of 1-day-old were ran-domly divided into 4 groups with 6 replicates per group and 12 broilers per replicate.Broilers in the control group(CON group)and LPS challenge group(LPS group)were fed a basal diet,and those in puerarin group(PUE group)and puerarin+LPS challenge group(PUEL group)were fed the basal diet supplemented with 750 mg/kg puerarin.The experiment period was 20 days.At 16,18 and 20 days of age,broilers in LPS group and PUEL group were intraperitoneally injected with 500 µg/kg BW LPS,and broilers in CON group and PUE group were intraperitoneally injected with the same amount of sterile saline.The results showed as fol-lows:1)LPS challenge extremely significantly decreased the average daily gain(ADG)of broilers(P＜0.01),tended to decrease the average daily feed intake(ADFI)(P=0.086)and increase the feed to gain ra-tio(F/G)(P=0.054).Dietary PUE tended to increase ADG of broilers(P=0.089).2)LPS challenge ex-tremely significantly decreased the total superoxide dismutase(T-SOD)activity in serum of broilers(P＜0.01),significantly decreased the catalase(CAT)activity in serum(P＜0.05),and extremely significantly increased the malondialdehyde(MDA)content in serum(P＜0.01).Dietary PUE significantly increased the T-SOD activity in serum(P＜0.05),and extremely significantly decreased the MDA content in serum(P＜0.01).3)LPS challenge extremely significantly increased the contents of interleukin-1 β(IL-1β),interleukin-2(IL-2),tumor necrosis factor-α(TNF-α),immunoglobulin A(IgA)and immunoglobulin G(IgG)in ser-um of broilers(P＜0.01),and extremely significantly decreased the interleukin-10(IL-10)content in serum(P＜0.01).Dietary PUE extremely significantly decreased the contents of IL-1β and IL-2 in serum(P＜0.01),and significantly decreased the IgG content in serum(P＜0.05).Dietary PUE and LPS challenge showed an extremely significant interaction on the serum IgA content(P＜0.01),and a significant interaction on the serum IgG content(P＜0.05).4)LPS challenge extremely significantly increased the contents of D-lac-tic acid(D-LA)and endotoxin in serum of broilers(P＜0.01).Dietary PUE and LPS challenge showed a sig-nificant interaction on the serum endotoxin content(P＜0.05).LPS challenge significantly decreased the ileal villus height(VH)and villus height to crypt depth ratio(V/C)of broilers(P＜0.05).5)LPS challenge and dietary PUE changed the cecal microbiota structure of broilers.At the phylum level,LPS challenge tended to decrease the Firmicutes relative abundance in cecum(P=0.078).At the genus level,LPS challenge extremely significantly decreased the relative abundances of Lactobacillus and norank_f_Eubacterium_coprostanoligenes_group in cecum(P＜0.01),and significantly increased the relative abundances of norank_f_norank_o_RF39 and norank_f_norank_o_Clostridia_vadinBB60_group in cecum(P＜0.05).Dietary PUE significantly in-creased the Butyricicoccus relative abundance in cecum(P＜0.05).Dietary PUE and LPS challenge showed an extremely significant interaction on the relative abundances of norank_f_norank_o_RF39 and Butyricicoccus in cecum(P＜0.01),and a significant interaction on the UCG-005 relative abundance in cecum(P＜0.05).In conclusion,dietary 750 mg/kg PUE can alleviate oxidative stress and inflammatory response,improve intesti-nal morphology and cecal microbiota structure,alleviate growth inhibition and adverse effects caused by LPS challenge to a certain extent.[Chinese Journal of Animal Nutrition,2024,36(8):5050-5066]
puerarinyellow-feathered broilerslipopolysaccharideantioxidantimmuneintestinal health
万方数据
维普
