This experiment was conducted to investigate the effects of dietary puerarin(PUE)on growth per-formance,serum antioxidant and immune indices,intestinal morphology and cecal microbiota of broilers chal-lenged with lipopolysaccharide(LPS).A total of 288 yellow-feathered male broilers of 1-day-old were ran-domly divided into 4 groups with 6 replicates per group and 12 broilers per replicate.Broilers in the control group(CON group)and LPS challenge group(LPS group)were fed a basal diet,and those in puerarin group(PUE group)and puerarin+LPS challenge group(PUEL group)were fed the basal diet supplemented with 750 mg/kg puerarin.The experiment period was 20 days.At 16,18 and 20 days of age,broilers in LPS group and PUEL group were intraperitoneally injected with 500 µg/kg BW LPS,and broilers in CON group and PUE group were intraperitoneally injected with the same amount of sterile saline.The results showed as fol-lows:1)LPS challenge extremely significantly decreased the average daily gain(ADG)of broilers(P＜0.01),tended to decrease the average daily feed intake(ADFI)(P=0.086)and increase the feed to gain ra-tio(F/G)(P=0.054).Dietary PUE tended to increase ADG of broilers(P=0.089).2)LPS challenge ex-tremely significantly decreased the total superoxide dismutase(T-SOD)activity in serum of broilers(P＜0.01),significantly decreased the catalase(CAT)activity in serum(P＜0.05),and extremely significantly increased the malondialdehyde(MDA)content in serum(P＜0.01).Dietary PUE significantly increased the T-SOD activity in serum(P＜0.05),and extremely significantly decreased the MDA content in serum(P＜0.01).3)LPS challenge extremely significantly increased the contents of interleukin-1 β(IL-1β),interleukin-2(IL-2),tumor necrosis factor-α(TNF-α),immunoglobulin A(IgA)and immunoglobulin G(IgG)in ser-um of broilers(P＜0.01),and extremely significantly decreased the interleukin-10(IL-10)content in serum(P＜0.01).Dietary PUE extremely significantly decreased the contents of IL-1β and IL-2 in serum(P＜0.01),and significantly decreased the IgG content in serum(P＜0.05).Dietary PUE and LPS challenge showed an extremely significant interaction on the serum IgA content(P＜0.01),and a significant interaction on the serum IgG content(P＜0.05).4)LPS challenge extremely significantly increased the contents of D-lac-tic acid(D-LA)and endotoxin in serum of broilers(P＜0.01).Dietary PUE and LPS challenge showed a sig-nificant interaction on the serum endotoxin content(P＜0.05).LPS challenge significantly decreased the ileal villus height(VH)and villus height to crypt depth ratio(V/C)of broilers(P＜0.05).5)LPS challenge and dietary PUE changed the cecal microbiota structure of broilers.At the phylum level,LPS challenge tended to decrease the Firmicutes relative abundance in cecum(P=0.078).At the genus level,LPS challenge extremely significantly decreased the relative abundances of Lactobacillus and norank_f_Eubacterium_coprostanoligenes_group in cecum(P＜0.01),and significantly increased the relative abundances of norank_f_norank_o_RF39 and norank_f_norank_o_Clostridia_vadinBB60_group in cecum(P＜0.05).Dietary PUE significantly in-creased the Butyricicoccus relative abundance in cecum(P＜0.05).Dietary PUE and LPS challenge showed an extremely significant interaction on the relative abundances of norank_f_norank_o_RF39 and Butyricicoccus in cecum(P＜0.01),and a significant interaction on the UCG-005 relative abundance in cecum(P＜0.05).In conclusion,dietary 750 mg/kg PUE can alleviate oxidative stress and inflammatory response,improve intesti-nal morphology and cecal microbiota structure,alleviate growth inhibition and adverse effects caused by LPS challenge to a certain extent.[Chinese Journal of Animal Nutrition,2024,36(8):5050-5066]
关键词
葛根素/黄羽肉鸡/脂多糖/抗氧化/免疫/肠道健康
Key words
puerarin/yellow-feathered broilers/lipopolysaccharide/antioxidant/immune/intestinal health
维普
万方数据