This experiment was conducted to investigate the protein metabolism rule and requirement of 500 to 550 kg Simmental hybrid beef cattle.Thirty healthy Simmental hybrid bulls with similar weight of(497.73±43.84)kg were randomly divided into 5 groups with 6 replicates per group and 1 cow per replicate.The pre-feeding period was 10 days,and the formal experiment period was 45 days.The comprehensive net energy of all diets was 6.12 MJ/kg.The crude protein(CP)levels of the diets were 9.53%,10.53%,11.53%,12.53%and 13.53%,respectively.The effects of different dietary CP levels on growth performance and pro-tein metabolism of Simmental hybrid cattle were studied through feeding experiment and digestion and metabo-lism experiment.A prediction model of protein requirement of 500 to 550 kg Simmental hybrid cattle was es-tablished.The results showed as follows:1)with the increase of dietary CP level,the average daily gain(ADG)of beef cattle in all groups was firstly increased and then decreased,and the ADG in 12.53%group was the highest,which was significantly higher than that in 9.53%and 13.53%groups(P<0.05),and the feed/gain(F/G)in 12.53%group was the lowest,which was significantly lower than that in 13.53%group(P<0.05).2)Different dietary CP levels had no significant effects on CP digestibility of 500 to 550 kg Sim-mental hybrid cattle(P>0.05).Fecal nitrogen excretion of beef cattle in 10.53%group were the highest,which was significantly higher than that in 9.53%and 12.53%groups(P<0.05),and CP deposition rate of beef cattle in 12.53%group was the highest,which was significantly higher than that in other groups(P<0.05).3)The CP requirement equation of 500 to 550 kg Simmental cross cattle was CP=7.89W0.75+68.60ADG,the maintenance CP(CPm)requirement was 7.89 g/(kg W0.75·d),and the CP per kilogram of weight gain(CPg)requirement was 68.60 g.The digestible CP(DCP)requirement equation is DCP=6.68W0.75+61.19ADG,the maintain digestible CP(DCPm)requirement is 6.68 g/(kgW0.75·d),and the di-gestible CP per kilogram of weight gain(DCPg)requirement is 61.19 g.Under the conditions of this experi-ment,the optimal dietary CP level for 500 to 550 kg Simmental hybrid bulls is 12.53%,and the results of this study can provide data reference for protein requirements of 500 to 550 kg Simmental hybrid bulls.[Chinese Journal of Animal Nutrition,2024,36(8):5085-5093]